高锌摄入对小鼠肝功能、肝药物代谢酶及膜流动性影响

小鼠用高锌饲料（含锌量１５ｇ／ｋｇ）连续喂养３周。结果显示，高锌可引起肝谷草转氨酶活性显著下降，肝糖元含量明显减少，对谷丙转氨酶及谷胱甘肽含量有减少的趋势，尚无显著影响。高锌对肝药物代谢酶活性影响各不相同，可使苯胺羟化酶活性明显升高，使谷胱甘肽Ｓ－转移酶，ＮＡＤＰＨ－细胞色素Ｃ还原酶及以甲基苯异丙基苄胺为底物的脱甲基酶活性明显下降，对细胞色素Ｐ４５０、细胞色素ｂ５及以氨基比林为底物的脱甲基酶活性无显著影响。此外，高锌能明显提高肝微粒体膜表层流动性，对深层膜流动性无明显影响。

Effects of High Dietary Zinc on Liver Function, Hepatic Drug Metabolism Enzymes and Membrane Fluidity in Mice

Mice were fed with high zinc diet (15 g/kg) for 3 weeks. High zinc could cause liver toxicity: 1. inhibiting the activity of GOT and GPT in liver homogenate, reducing GSH and glycogen contents. 2. increasing the activity of aniline hydroxylase and inhibiting the activities of NADPH-cytochrome C reducease, benzo-phytamine-N-demethylase and glutathione S-transferase. The activities of cytochrome P450 and cytochrome b5 were not obviously changed 3. increasing microsomal membrane fluidity in the superficial layers, but not in the deep layers.