铁超载与铁剥夺对HL-60细胞bcl-2和cmy-c基因的影响

目的探讨铁超载与铁剥夺对HL60细胞bc12和cmyc基因表达的影响。方法体外培养HL60细胞,分别加入不同浓度的三氯化铁(FeC13)。采用免疫组化LASB法测定凋亡相关基因bc12及cmyc表达。结果不同浓度的FeC13作用于HL60细胞6h、12h、24h、48h,bc12表达显著高于对照组。尤以100μmolLFeC13组显著,与对照组相比差异显著(P<0.05)。不同浓度DFO作用于HL60细胞一定时间后,其cmyc表达增高。结论铁超载时bc12高表达,铁剥夺时cmyc高表达。

The effect of iron-overload and iron-deprived on bcl-2 and c-myc expression in human leukemic HL-60 cells

Objective To investigate bc1-2 and c-myc Expression the Human Leukemic HL-60 Cells by iron, iron chelator. Methods HL-60 cells were cultured with different concentrations of iron(Ⅲ)chloride(FeC1_3),iron chelator-desferrioxamine(DFO)for 6h, 12h, 24h, 48h. They were divided into iron group(FeC1_3 5, 10, 20, 50, 100,150μmol/L, 0 μmol/L FeC1_3 as the control group),DFO group(DFO 10, 20, 50, 100, 150μmol/L , 0 umol/L DFO as the control group). The expression of C-myc or Bc1-2 by immunohistochemistry methods. Results The results showed that when HL-60 cells were incubated with different concentrations of FeC1_3 at 6h, 12h, 24h, 48h, the numbers of bc1-2 expression positive cells were much higher than that of control groups (P<0.05).When HL-60 cell swere incubated with DFO, the expression of C-myc on HL-60 cells in DFO group was higher than that of control group at 6h, 12h, 24h(P<0.05).Conclusions Iron-overload could induced over-expression of bc1-2.Iron-deprived could induced upregulation of c-myc expression in HL-60.