子宫内膜癌患者miR-30b和miR-96水平及与预后的关系

  目的  检测子宫内膜癌(endometrial cancer，EC)患者外周血单个核细胞(peripheral blood mononuclear cell，PBMC)miR-30b、miR-96水平，探究其与临床病理及预后的关系。  方法  选择2012年10月—2014年8月温州市中西医结合医院收治的EC患者及门诊健康体检者(对照组)各76例，荧光定量PCR检测PBMC中miR-30b、miR-96水平，Pearson法分析二者相关性，Kaplan-Meier法分析二者与5年生存率的关系；Cox分析不良预后的危险因素。  结果  EC患者PBMC中miR-30b、miR-96水平均高于对照组(t=21.913、24.188，均P＜0.05)，二者呈正相关(r=0.553，P＜0.05)，均与病理分级、FIGO分期、肌层浸润及淋巴结转移有关(均P＜0.05)。miR-30b高表达者5年总生存率(16.13%)低于低表达者(66.67%，χ2=16.890，P＜0.05)；miR-96高表达者5年总生存率(18.18%)低于低表达者(67.44%，χ2=16.307，P＜0.05)。miR-30b、miR-96、FIGO分期、淋巴结转移是预后的影响因素(HR=2.861, 95% CI: 1.427~5.736；HR=1.973, 95% CI: 1.462~2.663；HR=2.235, 95% CI: 1.491~3.350；HR=2.439, 95% CI: 1.365~4.358；均P＜0.05)。  结论  PMBC中miR-30b、miR-96高表达，与EC患者病理分级、FIGO分期、肌层浸润、淋巴结转移及5年生存率有关，是影响预后的独立危险因素。 

Expression of miR-30b and miR-96 in endometrial carcinoma and their relationship with prognosis

Objective The levels of miR-30 b and miR-96 in peripheral blood mononuclear cells(PBMC)in patients with endometrial carcinoma(EC)were detected to explore their relationship with clinicopathology and prognosis.Methods A total of 76 EC patients and healthy outpatients(control group)admitted to Wenzhou hospital of integrated traditional Chinese and western medicine were selected.The expression levels of miR-30 b and miR-96 in PBMC were detected by fluorescence quantitative PCR,the correlation was analyzed by Pearson method,the relationships between miR-30 b and miR-96 expression levels in PBMC and 5-year survival rate of EC patients were analyzed by Kaplan-Meier method,and the risk factors affecting the prognosis of EC patients were analyzed by Cox regression.Results The expression levels of miR-30 b and miR-96 in PBMC of EC patients were higher than those of control group(t=21.913,24.188,all P<0.05).They were positively correlated(r=0.553,P<0.05),and correlated with pathological grade,FIGO stage,depth of muscle invasion and lymph node metastasis(P<0.05).The 5-year overall survival rate of patients with high expression of miR-30 b(16.13%)was lower than that of patients with low expression(66.67%,χ²=16.890,P<0.05).The 5-year overall survival rate of patients with high expression of miR-96(18.18%)was lower than that of patients with low expression(67.44%,χ²=16.307,P<0.05).Levels of miR-30 b and miR-96,FIGO stage and lymph node metastasis in PMBC were independent risk factors affecting the prognosis of EC patients(HR=2.861,95%CI:1.427-5.736;HR=1.973,95%CI:1.462-2.663;HR=2.235,95%CI:1.491-3.350;HR=2.439,95%CI:1.365-4.358;all P<0.05).Conclusion The miR-30 b and miR-96 are highly expressed in PMBC,which are related to pathological grade,FIGO stage,muscle invasion,lymph node metastasis and 5-year survival rate of EC patients,and are independent risk factors for adverse prognosis of EC patients.