miR-497通过靶向调节YAP1表达对甲状腺乳头状癌细胞生物学行为的影响

目的:探讨miR-497通过靶向调节Yes相关蛋白1（Yes-associated protein 1，YAP1）表达对甲状腺乳头状癌（papillary thyroid carcinoma，PTC）细胞生物学行为的影响。方法:将人PTC细胞株TPC-1细胞分为对照组、miR-497组、si-YAP1组和miR-497...

Effects of miR-497 on cytobiology behaviors of papillary thyroid carcinoma by targetingly regulating YAP1 expression

Objective To investigate the effects of miR-497 on cytobiology behaviors of papillary thyroid carcinoma(PTC)by targetingly regulating the expression of Yes-associated protein 1(YAP1).Methods Human TPC-1 cells were divided into control group.miR-497 group,si-YAPl group and miR-497+si-YAPl group.The liposome transfection was conducted by LipofectamineTM3000.The targeted relationship between miR-497 and YAP1 was validated by Luciferase Reporter Assay.The cell proliferation activity in each group was detected by MTT method.The apoptosis rates were analyzed by flow cytometry.The number of invasion cells was detected by Transwell.The cell migration rates were detected by scratch assay.The expression of Cyclin DI.matrix metallo proteinase 2(MMP-2),MMP-9,matrix metalloproteinase inhibitor-1(TIMP-1)and activated caspase 3(cleaved Caspase-3)was detected by Western blot.SPSS 22.0 was used to analyze data,and normally distributed measurement data were expressed as(x±s).One-way ANOVA was analyzed for the difference between multiple groups,and SNK-q were analyzed for the difference between two groups.Results Compared with the control group,the expression of YAP 1 mRNA and protein was decreased in miR-497 group and si-YAPl group(q=14.682,14.597;23.743,23.571;P<0.05),cell proliferation activity,number of invasion cells and migration rate were decreased(q=4.724,4.56&3.841;4.216,3.952,3.274;P<0.05),apoptosis rate was increased(q=3.783;4.336;P<0.05),expression of CyclinDl,MMP-2,MM P-9 and cleared Caspase-3 proteins was decreased(q=5.823,5.981,6.036,6.485;5.934,6.110,6.573,6.614;P<0.05),and expression of TIMP-1 protein was increased(q=6.071;6.148;P<0.05).Compared with si-YAP1 group,miR-497 level was increased in miR-497+si-YAPl group(q=14.726,P<0.05),the expression of YAP1 mRNA and protein was decreased(q=3.089,3.126;P<0.05),cell proliferation activity,number of invasion cells and migration rate were decreased(g=2.654,2.537,2.246;P<0.05),apoptosis rate was increased(q=2.875,P<0.05),expression of CyclinDl,MMP-2,MM P-9 and cleared Caspase-3 proteins was decreased(q=4.371,4.365,4.383,4.368;P<0.05),and expression of TIMP-1 protein was increased(q=4.275,P<0.05).Conclusion MiR-497 can negatively targetingly regulate the expression of YAP1,inhibit proliferation,invasion and migration of TPC-1 cells.