Protective Effects of Pine Bark Extract on Hexavalent Chromium‐Induced Dermatotoxicity in Rats |
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Authors: | In‐Chul Lee Sung‐Hwan Kim In‐Sik Shin Changjong Moon Soo‐Hyun Park Sung‐Ho Kim Seung‐Chun Park Hyoung‐Chin Kim Jong‐Choon Kim |
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Institution: | 1. College of Veterinary Medicine, Chonnam National University, , Gwangju, 500‐757 South Korea;2. Herbal Medicine EBM Research Center, Korea Institute of Oriental Medicine, , Daejeon, 305‐811 South Korea;3. College of Veterinary Medicine, Kyungpook National University, , Daegu, 702‐701 South Korea;4. Biomedical Mouse Resource Center, Korea Research Institute of Bioscience and Biotechnology, , Chungbuk, 363‐883 South Korea |
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Abstract: | The present study investigated the protective effects of pine bark extract (PBE) against hexavalent chromium Cr(VI)]‐induced dermatotoxicity in rats. Skin reactions were evaluated by visual inspection, histopathological changes and oxidative stress parameters. Topical application of Cr(VI) produced a significant increase in the incidence and severity of erythema and edema upon visual inspection. Histopathological examination showed moderate to severe necrosis and desquamation in the epidermis and inflammation and hemorrhage in the dermis. In addition, an increased malondialdehyde (MDA) concentration, and decreased glutathione (GSH), catalase, superoxide dismutase, glutathione‐S‐transferase (GST) and glutathione reductase of the skin were observed in the Cr(VI) group. On the contrary, concomitant administration with PBE significantly improved Cr(VI)‐induced dermatotoxicity, evidenced by a decrease in the incidence and severity of skin irritation and histopathological lesions in a dose‐dependent manner. Moreover, PBE treatment reduced MDA concentrations and increased catalase and GST activities in skin tissues, indicating that concomitant administration with PBE effectively prevents Cr(VI)‐induced oxidative damage in rats. The results indicate that PBE has a protective effect against Cr(VI)‐induced dermatotoxicity and is useful as a protective agent against various dermal lesions induced by oxidative stress. Copyright © 2012 John Wiley & Sons, Ltd. |
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Keywords: | hexavalent chromium dermatotoxicity pine bark extract oxidative stress protective effect |
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