Piperine Inhibits Lipopolysaccharide‐induced Maturation of Bone‐marrow‐derived Dendritic Cells Through Inhibition of ERK and JNK Activation |
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Authors: | Gi‐Sang Bae Jong‐Jin Kim Kyoung‐Chel Park Bon Soon Koo Il‐Joo Jo Sun Bok Choi Chang Hyuk Lee Won‐Seok Jung Jung‐Hee Cho Seung‐Heon Hong Ho‐Joon Song Yong Kook Shin Sung‐Joo Park |
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Affiliation: | 1. Hanbang Body‐fluid Research Center, Wonkwang University, , Iksan, 540‐749 Jeonbuk, South Korea;2. ChungBuk Technopark Bio Center, , Jecheon, ChungBuk, 390‐250 South Korea;3. Department of Herbology, School of Oriental Medicine, Wonkwang University, , Iksan, Jeonbuk, 540‐749 South Korea;4. Jeollanamdo Development Institute for Korean Traditional Medicine, , Jangheung, Jeollanamdo, 529‐851 South Korea;5. Wonkwang Oriental Medicines Research Institute, Department of Oriental Pharmacy, Wonkwang University, , Iksan, Jeonbuk, 540‐749 South Korea |
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Abstract: | Piperine, one of the main components of Piper longum Linn. and P. nigrum Linn., is a plant alkaloid with a long history of medicinal use. Piperine has been shown to modulate the immune response, but the mechanism underlying this modulation remains unknown. Here, we examined the effects of piperine on lipopolysaccharide (LPS)‐induced inflammatory responses in bone‐marrow‐derived dendritic cells (BMDCs). Piperine significantly inhibited the expression of major histocompatibility complex class II, CD40 and CD86 in BMDCs in a dose‐dependent manner. Furthermore, piperine treatment led to an increase in fluorescein‐isothiocyanate–dextran uptake in LPS‐treated dendritic cells and inhibited the production of tumour necrosis factor alpha and interleukin (IL)‐12, but not IL‐6. The inhibitory effects of piperine were mediated via suppression of extracellular signal‐regulated kinases and c‐Jun N‐terminal kinases activation, but not p38 or nuclear factor‐κB activation. These findings provide insight into the immunopharmacological role of piperine. Copyright © 2012 John Wiley & Sons, Ltd. |
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Keywords: | piperine dendritic cells lipopolysaccharide dextran |
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