2006年广西流行性出血热宿主动物标本检测结果分析

目的对广西部分地区的肾综合征出血热宿主动物标本进行病毒学检测。方法2006年在广西部分地区采集鼠血及鼠肺标本,用ELISA法测定其抗原和抗体。对汉坦病毒抗原阳性标本通过RT-PCR法扩增部分M片段上的核苷酸序列并测序,将扩增片段的核苷酸序列与已知病毒序列进行比对并分型。结果在294份褐家鼠、黄胸鼠、小家鼠鼠血标本中有42份抗体阳性,鼠抗体阳性率为14.3%,在鼠肺标本中检测到一株SEO型的出血热病毒,带毒率为0.33%,阳性标本来源为广西沿海地区钦州市。结论2006年捕获的宿主动物有褐家鼠、黄胸鼠、小家鼠等,鼠抗体阳性率为14.3%,带毒率为0.33%,鼠抗体阳性率和带毒率明显低于全国平均水平;鉴定出一株SEO型汉坦病毒。

Detection of rodent specimen from epidemic hemorrhagic fever in Guangxi

Objective To detect HFRSV from rats in some BI Fu-yin, TAN Yi, WEI Contorl and Prevention, Nanning 530021, China areas of Guangxi.Methods Rats were collected fromsome areas of Guangxi and Hantanvirus in lung and antibody in blood were detected by ELISA. M segment of positive specimen was partially amplified with RT- PCR and sequenced. Phylogenetic analysis was conducted for genotyping. Results There were 42 positive in 294 blood specimens and 1 in 306 lung specimens. A strain of SEO hantavirus was detected from lung specimens of a Norvegicus rat from coastal area of Guangxi Qinzhou City. Concluslons Rattus norvegicus,Rattus flavipectus and Mus musculus were catched in 2006. Positive rate of antibody in blood was 14.3% and positive rate of antigen in lung was 0.33% ,they were lower than the level of average rate in China. A strain of SEO hantavirus was detected in Guangxi.