Neospora caninum surface antigen (p40) is a potential diagnostic marker for cattle neosporosis |
| |
Authors: | Pengfei He Jianhua Li Pengtao Gong Chengwu Liu Guocai Zhang Ju Yang Wenbin Tuo Bintong Yang Xichen Zhang |
| |
Affiliation: | 1. College of Animal Science and Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, People’s Republic of China 2. Shengyang Policedog Training School, 4 BaiShan Road, Shenyang, 110034, China 3. Animal Parasitic Diseases Laboratory, Beltsville Agricultural Research Center, USDA/ARS, Beltsville, MD, 20705, USA 4. College of Animal Science and Technology, Jilin Agricultural University, 2888 Xinchen Street, Changchun, China
|
| |
Abstract: | Neospora caninum is an intracellular protozoan that infects domestic and wild canids as well as many warm-blooded animals as shown by the isolation of viable parasites. The effectiveness of diagnostic tests for detecting specific antibodies against N. caninum is hampered by potential cross-reaction with other Coccidia. So, there is currently an urgent need for a sensitive and specific diagnostic assay for detecting N. caninum in animals. The N. caninum 40-kD surface antigen (p40), similar to NcSAG1 and NcSRS2, was shown to belong to surface antigen super family and thus represents an excellent marker for the diagnosis of neosporosis. In order to test the hypothesis, recombinant Ncp40 (rNcp40) was expressed in Escherichia coli, and an indirect ELISA test was developed using recombinant NCp40 antigen for N. caninum serodiagnosis. The antigen used in this study did not have cross-reactivity with anti-Toxoplasma gondii serum. Anti-p40 antibodies were detected by ELISA in the sera of Yellow cattle and were compared with (IFAT). Optimal sensitivity and specificity (98.2 and 98.6 %) were identified by IFAT. Additionally, 37 positive sera of T. gondii were detected and there was no significant difference with the negative serum of N. caninum. The rNcp40 ELISA developed here provides a specific and sensitive assay for detecting neosporosis in cattle. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|