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| 贲门癌组织GSTM1,GSTT1和GSTP1基因多态变化 | |
| 引用本文： | 周琦,郑作昱,王立东,益新娜,王道存,常志伟,刘宾,李吉林.贲门癌组织GSTM1,GSTT1和GSTP1基因多态变化[J].郑州大学学报(医学版),2003,38(3):327-329. |
| 作者姓名： | 周琦 郑作昱 王立东 益新娜 王道存 常志伟 刘宾 李吉林 |
| 作者单位： | 1. 郑州大学医学院癌症研究室,郑州,450052 2. 首都医科大学同仁医院消化内科,北京,100013 3. 林州市姚村食管癌医院病理科,林州,456592 |
| 基金项目： | 国家杰出青年科学基金3 0 025016，河南省高校创新人才工程基金，河南省医药卫生创新人才工程基金，国家自然科学基金资助项目 39770 2 96 |
| 摘 要： | 目的：探讨河南林州地区贲门癌组织中3种Ⅱ期代谢酶基因GSTMl，GSTTl及GSTPl基因多态性变化，进一步深入了解该地区贲门癌高易感性分子基础。方法：利用multlplex—PCR、PCR—RFLP检测19例手术切除贲门腺癌组织及72例正常对照组织(颊粘膜细胞和血细胞)中代谢酶基因GSTMl、GSTT1、GSTPl多态性改变。结果：贲门腺癌与正常对照组织中GSTMl纯合缺失基因型分别占37％和39％，该基因型未明显增加对贲门癌的易感性(0R＝0．69)；GSTT1纯合缺失基因型分别占53％和39％，该基因型可能与贲门癌易感性增高有关(0R＝2．38)；GSTPl的IIe／Val和Val／Val基因型合并在一起分别占26％和42％，Val等位基因能降低个体贲门癌的易感性(0R＝0．41)。结论：GSTMl基因多态对贲门癌易感性无明显影响，而GSTTl和GSTPl基因多态与贲门癌易感性有关。 |
| 关 键 词： | 贲门癌 GSTMl GSTTl GSTPl 基因多态 癌组织 |
| 修稿时间： | 2003年2月20日 |
Prevalence of genetic polymorphisms of GSTM1, GSTT1 and GSTP1 in subjects with gastric cardia adenocarcinoma at Linzhou, Henan |
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| ZHOU Qi ,ZHENG Zuoyu ,WANG Lidong ,YI Xinna ,WANG Daocun ,CHANG Zhiwei ,LIU Bin ,LI Jilin Laboratory for Cancer Research,College of Medicine,Zhengzhou University,Zhengzhou.Prevalence of genetic polymorphisms of GSTM1, GSTT1 and GSTP1 in subjects with gastric cardia adenocarcinoma at Linzhou, Henan[J].Journal of Zhengzhou University: Med Sci,2003,38(3):327-329. | |
| Authors: | ZHOU Qi ZHENG Zuoyu WANG Lidong YI Xinna WANG Daocun CHANG Zhiwei LIU Bin LI Jilin Laboratory for Cancer Research College of Medicine Zhengzhou University Zhengzhou |
| Institution: | ZHOU Qi 1),ZHENG Zuoyu 1),WANG Lidong 1),YI Xinna 1),WANG Daocun 1),CHANG Zhiwei 1),LIU Bin 2),LI Jilin 3) 1)Laboratory for Cancer Research,College of Medicine,Zhengzhou University,Zhengzhou 450052 2)Department of Gastroenterolgy,Tongren Hospital,Capital Medical University,Beijing 100013 3)Department of Pathology,Yaocun Esophageal Cancer Hospital,Linzhou 456592 |
| Abstract: | Aim: To explore the main molecular alterations in gastric cardia adencarcinoma (GCA) related with the high susceptibility of GCA in the population in Linzhou, China. Methods: PCR and PCR RFLP methods were performed to determine the polymorphisms of metabolizing enzymes in 19 GCA samples and 72 normal controls samples (buccal smear or blood sample).Results: (1) The frequencies of GSTM1 null genotype in tumor group and normal controls were 37% and 39%, respectively. The GSTM1 null genotype did not change the susceptibility to GCA ( OR =0.69); (2) The frequencies of GSTT1 null genotype in tumor group and normal controls were 53% and 39% respectively. This genotype may increase the susceptibility to GCA ( OR =2.38); (3) The combined frequencies of Ile/Val and Val/Val genotype of GSTP1 in tumors and normal controls were 26% and 42% , respectively. The Val allele decreased the susceptibility to GCA ( OR =0.41). Conclusion: The polymorphisms of GSTM1 did not significantly change the susceptibility to GCA. However, the polymorphisms of GSTT1and GSTP1 might be associated with the susceptibility to GCA. |
| Keywords: | Gastric cardia adenocarcinoma (GCA) Genetic polymorphisms GSTM1 GSTT1 GSTP1 |
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