| 顺铂、氧化苦参碱对肝癌QGY细胞生长及端粒酶活性的影响 |
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| 引用本文： | 何松,左国庆,张燕,汤为学.顺铂、氧化苦参碱对肝癌QGY细胞生长及端粒酶活性的影响[J].重庆医科大学学报,2006,31(5):677-680. |
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| 作者姓名： | 何松 左国庆 张燕 汤为学 |
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| 作者单位： | 1. 重庆医科大学附属第二医院消化内科,重庆,400010
2. 基础医学院病理生理学教研室,重庆,400016 |
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| 摘 要： | 目的：观察顺铂、氧化苦参碱对人肝癌细胞株QGY端粒酶活性的影响，进一步探讨两种药物的可能作用机制。方法：采用人肝癌细胞株QGY作为研究对象，MTT法检测不同浓度和作用时间的药物对细胞增殖的抑制作用，以及对QGY细胞粘附能力的影响；流式细胞仪分析细胞周期的分布及凋亡；PCR—ELISA法检测端粒酶活性的变化。结果：两种药物均具有抗QGY细胞增殖、阻滞细胞周期、诱导凋亡及抑制端粒酶活性的作用。结论：对端粒酶活性的抑制可能是两种药物发挥抗肿瘤作用的机制之一。
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| 关 键 词： | 肝细胞癌 端粒酶 顺铂 氧化苦参碱 |
| 文章编号： | 0253-3626(2006)05-0677-04 |
| 收稿时间： | 03 6 2006 12:00AM |
| 修稿时间： | 2006年3月6日 |
Effects of cisplatin, oxymatrine on the cell growth and telomerase activity of QGY hepatoma cells |
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| HE Song, et al.Effects of cisplatin, oxymatrine on the cell growth and telomerase activity of QGY hepatoma cells[J].Journal of Chongqing Medical University,2006,31(5):677-680. |
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| Authors: | HE Song |
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| Institution: | Department of Gastroenterology, the Second Affiliated Hospital, Chongqing Medical University |
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| Abstract: | Objective:To investigate the anti-cancer mechanism of cisplatin,oxymatrine by observing their effects on telomerase activity.Methods:The inhibition of proliferation in QGY cells were estimated by MTT-test.Distribution of cell cycle and apoptosis were analysed using flow cytometry.The changes of telomerase activity were detected by PCR-ELISA method.Results:These two drugs could inhibit the proliferation of QGY cells,block the cell cycle,induce its apoptosis and inhibit the activity of telomerase.Conclusion:The inhibition of telomerase activity may be one of the mechanisms for the anti-cancer effect of the two drugs. |
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| Keywords: | Hepatocellular carcinoma Telomerase Cisplatin Oxymatrine |
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