WHBE兔痰瘀证心肌缺血模型的建立

目的利用高脂喂养结合冠脉结扎建立WHBE兔痰瘀证心肌缺血模型。方法 18只WHBE兔随机分成正常对照(NC)组、心肌缺血模型(MI)组和痰瘀证心肌缺血模型(PBSS-MI)组,每组6只,MI组和PBSS-MI组均采用冠脉结扎法建立心肌缺血,然后NC组和MI组饲喂普通饲料,PBSS-MI组饲喂高脂饲料,连续造模4周,制备痰瘀证心肌缺血模型,分别通过测定动物的体重、心肌酶指标、血脂水平、血液流变学和心肌梗死面积(MIS)来评价模型的建立和痰瘀证的病理进程,用Pearson法进行相关性分析。结果与NC组比,MI组体重略有降低,但血清CK、LDH、TG、血浆粘度、卡松粘度和心肌梗死面积均明显升高(P0.05,P0.01),PBSS-MI组体重降低不明显,但血清CK、LDH、TC、TG、LDL-C、HDL-C、全血粘度、血浆粘度、红细胞电泳时间、卡松粘度、心肌梗死面积均显著升高(P0.05,P0.01);与MI组比,PBSS-MI组TC、LDL-C、HDL-C、高切全血粘度、红细胞电泳时间和心肌梗死面积均高于MI组(P0.05,P0.01)。相关分析显示,MI组MIS与TC、TG和HDL-C指标密切相关(P0.01);而PBSS-MI组MIS与TC、TG、HDL-C、LDL-C、LDH、CK、高切和低切全血粘度、红细胞电泳时间和卡松粘度密切相关(P0.05,P0.01)。结论高脂喂养结合冠脉结扎法在WHBE兔体内可成功建立痰瘀证心肌缺血模型,且缺血程度与血脂、血液流变学有相关性变化,这些可反映了中医"痰瘀互结"的相关生物学基础。

Establishment of Myocardial Ischemia Model of Phlegm-blood Stasis Syndrome Type in WHBE Rabbits

Abstract] Objective To establish the disease syndrome combined animal model, the WHBE rabbit myocardial ischemia model of phlegm-blood stasis syndrome type, by high fat diet feeding and coronary artery ligation. Methods WHBE rabbits were randomly divided into the normal control (NC) group, myocardial ischemia model (MI) group and myocardial ischemia model of phlegm-blood stastis syndrome (PBSS-MI) group, 6 in each group. The myocardial ischemia of MI group and PBSS-MI group were induced by ligated the coronary artery, and then NC group and MI group were fed with common forage, while PBSS-MI group was fed with high fat forage for 4 weeks. The model establishment and its pathological process of phlegm-blood stasis syndrome were evaluated by examinations on body weight, myocardial enzymes indexes, blood liquid levels, hemorheology and myocardial infarction size (MIS), and correlation analysis by Pearson method. Results Compared with NC, the weight of MI group was slightly lowered, and the serum CK, LDH, TG, plasma viscosity, casson viscosity and myocardial infarction size were significantly increased (P<0.05, P<0.01).While the weight of PBSS-MI group had no obviously lowered, but serum CK, LDH, TC,TG, LDL-C, HDL-C, whole blood viscosity, plasma viscosity, erythrocyte electrophoresis time, casson viscosity and myocardial infarction size were significantly increased (P<0.05, P<0.01). Compared with MI group, TC, LDL-C, HDL-C, high shear rate of whole blood viscosity, erythrocyte electrophoresis time and myocardial infarction size were higher than those of MI group (P<0.05, P<0.01). Correlation analysis showed that MIS and TC, TG and HDL-C in MI group were closely related (P<0.01), and MIS and TC,TG, HDL-C, LDL-C, LDH, CK, high shear rate of whole blood viscosity, low shear rate of whole blood viscosity, erythrocyte electrophoresis time and cassion viscosity in PBSS-MI group were also closely related (P<0.05, P<0.01). Conclusion Disease syndrome combined model for myocardial ischemia model of phlegm-blood stasis syndrome type in WHBE rabbit could be established by high fat diet feeding with coronary artery ligation, and it has related changes between MIS and blood lipids level, hemorheology, which may reflect related biological basis of the traditional Chinese medicine of phlegm and stasis cementation.