Resolution of salbutamol enantiomers in human urine by reversed-phase high performance liquid chromatography after derivatization with 2,3,4,6-tetra-O-acetyl-β-D-glycopyranosyl isothiocyanate |
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Authors: | Kyeong Ho Kim Tae Kyun Kim |
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Institution: | 1. College of Pharmacy, Kangwon National University, 200-701, Chunchon, Korea 2. Institute of Life Science Research, Choseon Pharm. & Trading Co., 425-120, Ansan, Korea
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Abstract: | A stereospecific HPLC method has been developed for the resolution of the enantiomers of salbutamol in human urine. After solid-phase extraction and derivatization with 2,3,4,6-tetra-O-acetyl-β-D-glucopyranosyl isothiocyanate, the diastereomeric derivatives were resolved (Rs=1. 83) on 5 μm octadecylsilan column using 35% acetonitrile in 0.05M ammonium acetate buffer (pH=6) as a mobile phase with electrochemical detection. The diastereomeric derivatives were formed within 30 min. The detection limit of each enantiomer was 20 ng/ml (S/N=3). |
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