肝舒胶囊治疗二甲亚硝胺致大鼠肝纤维化的疗效研究

目的 研究肝舒胶囊（GSC）对二甲亚硝胺（DMN）致大鼠肝纤维化的治疗效果。方法 采用DMN大鼠肝纤维化模型,研究GSC对大鼠肝脾指数、肝脏组织形态学、肝组织羟脯氨酸（Hyp）含量以及血清透明质酸（HA）、层粘连蛋白（LN）、Ⅳ胶原（Ⅳ-C）和Ⅲ型前胶原（PCⅢ）的影响。结果 GSC高、中剂量组均能显著降低大鼠肝、脾指数以及肝组织Hyp含量（P〈0.01）,同时还可以降低大鼠血清HA、LN、IV-C和PCIII的水平（P〈0.01）;GSC高剂量组降低肝指数的作用明显优于秋水仙碱组（P〈0.01）;GSC还具有改善肝脏组织形态的作用。结论 GSC具有良好的治疗肝纤维化效果。

Study on the Effect of Ganshu Capsule on Treating Liver Fibrosis in Rats Induced by Dimethylnitrosamine

Abstract: objective: to study the effect of Ganshu capsule (GSC)on treating the liver fibrosis of rats induced byDMN(dimethylnitrosamines) .Methods: The animal model of liver fibrosis in rats was induced with DMN. The liver and spleen index, liver tissue morphology and hydroxyproline content were observed .And radioimmunoassay was used to detect hyaluronic acid (HA), laminin protein (LN), IV collagen (IV-C) and pre-collagen type III (PCIII). Results: The high and middle dose group of GSC not only could reduced liver, spleen index and liver hydroxyproline content significantly, but also could reduce serum HA, LN, IV-C and PCIII level significantly in rats (P < 0.01); Among them, the high dose group of GSC reduced the liver index significantly better than colchicine group(P < 0.01); GSC can also improve liver tissue morphology. Conclusion: GSC has a good effect on the treatment of liver fibrosis.