荧光测定法定量测定滤纸片干血斑标本G6PD酶活性的可靠性分析

目的探讨荧光测定法测定滤纸片干血斑标本G6PD酶活性的可靠性。方法随机选取43例门诊孕前咨询者为检测对象,每人采集2ml静脉血抗凝保存同时制备滤纸片干血斑标本。采用G6PD/6PGD比值法测定抗凝血G6PD/6PGD比值,同时采用荧光测定法定量测定滤纸片干血斑标本G6PD酶活性,比较两种方法测定结果。结果荧光测定法检测43份滤纸片干血斑标本,检出1例缺乏（1.57 IU/gHb）;比值法检测43份抗凝血标本,检出1例缺乏（0.76）;两种方法符合率为100%。结论荧光测定法定量测定G6PD酶活性准确性高、简单、快捷、费用低廉,可对滤纸片干血斑标本进行G6PD缺乏症的大规模筛查,适于在G6PD缺乏高发区推广应用。

The credibility of quantitive fluorometric assay for G6PD activity in dried blood spot specimens on filter paper

Objective:To investigate the credibility of quantitive fluorometric assay for glucose-6-phosphate dehydrogenase(G6PD) activity in dried blood spot specimens on filter paper.Methods: Randomly choosing 43 progestational consultants,each was collected 2ml venous blood in anticoagulant tubes, and dried blood spot specimen on filter paper was made at the same time.The fluorometric assay was used to quantitive detect G6PD activity in the dried blood spot specimens on filter paper,the G6PD/6GPD ratio of each anticoagulant blood sample was detected by G6PD/6GPD test kit,and compared with the results which detected by the two methods.Results: One case detected by fluorometric assay had enzyme deficiency(1.57IU/gHb),and the same case detected by G6PD/6GPD ratio test also had enzyme deficiency(0.76).The related coefficient of fluorometric assay with G6PD/6GPD ratio test for G6PD deficiency was 100%.Conclusion: The fluorometric assay is an accurate,high-throughout,quantitive and simple method for G6PD activity testing.It is very favorable to employ in high incidence districts of G6PD deficiency.