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| 丹参对ox-LDL孵育的乳鼠心肌细胞缺氧-复氧所致细胞凋亡的保护作用 | |
| 引用本文： | 张卓然,王鲁川,李鸿珠,郭津,王丽娜,张伟华,徐长庆.丹参对ox-LDL孵育的乳鼠心肌细胞缺氧-复氧所致细胞凋亡的保护作用[J].中医药学报,2009,37(5). |
| 作者姓名： | 张卓然 王鲁川 李鸿珠 郭津 王丽娜 张伟华 徐长庆 |
| 作者单位： | 1. 哈尔滨体育学院,黑龙江,哈尔滨,150080;哈尔滨医科大学病理生理教研室,黑龙江,哈尔滨,150086 2. 佳木斯大学第一附属医院骨外科,黑龙江,佳木斯,154003 3. 哈尔滨医科大学病理生理教研室,黑龙江,哈尔滨,150086 4. 哈尔滨医科大学病理生理教研室,黑龙江,哈尔滨,150086;黑龙江生物医药工程重点实验室,黑龙江,哈尔滨,150086 |
| 基金项目： | 国家自然科学基金资助项目，教育部高校博士点基金，哈尔滨医科大学研究生创新基金资助项目，黑龙江省研究生创新科研资金项目 |
| 摘 要： | 目的:观察丹参对ox-LDL孵育的乳鼠心肌细胞缺氧-复氧损伤的保护作用,并探讨其可能机制。方法:采用原代培养乳鼠的心肌细胞,ox-LDL孵育24小时,再采用95%N2+5%CO2模拟缺氧培养3小时后,更换为正常DMEM培养液培养9小时的方法建立心肌细胞缺氧/复氧模型。乳鼠心肌细胞随机分为6组:正常对照组(Control group);ox-LDL组(ox-LDL group);ox-LDL缺氧-复氧组(ox-LDL+A/R group);丹参保护组,加丹参低剂量(SM L/ox-LDL+A/R group)、中剂量(SM M/ox-LDL+A/Rgroup)、高剂量(SM H/ox-LDL+A/R group)组。利用MTT比色法检测心肌细胞存活率,Hoechst33342染色检测凋亡率,采用Western blot分别观察Cytc、Bax、bcl-2、caspase-3的蛋白表达。结果:通过MTT比色法检测,加入丹参高剂量组对细胞保护最有效。与Control组比较,ox-LDL组:细胞核有固缩和断裂,Cytc、Bax和caspase-3的表达升高,细胞存活率和bcl-2表达降低;与ox-LDL组比较,ox-... |
| 关 键 词： | 丹参 心肌细胞 缺氧-复氧 细胞凋亡 |
Protective Effect of Salvia Miltiorrhiza on Apoptosis of ox-LDL Cultured Myocardial Cells Induced by Anoxia/reoxygenation in Neonate Rat |
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| ZHANG Zhuo-Ran,WANG Lu-chuan,LI Hong-zhu,GUO Jin,WANG Li-na,ZHANG Wei-hua,XU Chang-Qing.Protective Effect of Salvia Miltiorrhiza on Apoptosis of ox-LDL Cultured Myocardial Cells Induced by Anoxia/reoxygenation in Neonate Rat[J].Acta Chinese Medicine and Pharmacology,2009,37(5). | |
| Authors: | ZHANG Zhuo-Ran WANG Lu-chuan LI Hong-zhu GUO Jin WANG Li-na ZHANG Wei-hua XU Chang-Qing |
| Institution: | 2; 41.Harbin institute of Physical Education; Harbin 150080; China; 2.Department of Pathophysiology; Harbin Medical University; Harbin 150086; 3.First Affiliated Hospital to Jiamusi University; Jiamusi 154003; 4.Bio-pharmaceutical Key Laboratory of Heilongjiang Province; |
| Abstract: | Objective:To observe protective effect of Salvia on ox-LDL cultured myocardial cells injured by anoxia/reoxygenation in neonate rat.Methods:The rat myocardial atherosclerosis cellular models were prepared using the primary cultured myocardial cells incubated with ox-LDL;the myocardial models of anoxia/reoxygenation:cells first were maintained in a humidified 37℃,95%N2-5%CO2 incubator for 3 hours,then replaced culture fluid with DMEM and cultured in normal condition for 9 hours.Cells of neonate rat were divi... |
| Keywords: | Salvia miltiorrhiza Myocardial cells Atherosclerosis anoxia/reoxygenation Apoptosis |
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