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Molecular epidemiology of methicillin-resistant Staphylococcus aureus
Authors:Shopsin B  Kreiswirth B N
Affiliation:Public Health Research Institute and Department of Microbiology, New York University School of Medicine, New York, New York, USA.
Abstract:Subtyping methicillin- resistant Staphylococcus aureus (MRSA) isolates and tracking nosocomial infections have evolved from phenotypic to genotypic approaches; most laboratories now depend on pulsed-field gel electrophoresis (PFGE). We discuss the limitations of current image-based genotyping methods, including PFGE, and the advantages (including ease of entering data into a database) of using DNA sequence analysis to control MRSA infections in health-care facilities.
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