KLF8在不同肝癌细胞株及肝癌组织中的表达及其意义

目的 观察不同肝癌细胞株及肝癌组织中Krappel样因子8(KLF8)的表达,并探讨其意义.方法 用逆转录.聚合酶链反应(RT-PCR)、Western blot、免疫化学方法分别检测不同肝癌细胞株及肝(癌)组织中KLF8的mRNA和蛋白质水平.结果 KLF8的mRNA及蛋白质水平随肝癌细胞株侵袭转移潜能的增强而明显升高(P<0.05);正常肝组织(6例)、无转移肝癌组织(17例)、有转移肝癌组织(6例)中KLF8 mRNA的相对表达量分别为0.2377±0.0658、0.4683±0.2073、0.6669±0.0239(P<0.05);在正常肝组织和肝硬化组织中KLF8蛋白表达的阳性率分别为16.7%(1/6)和33.3%(2/6),均为弱阳性,在肝癌组织中强阳性率为81.8%(27/33),肝癌转移灶中强阳性率为83.3%(5/6)(P<0.01).结论 KLF8可能参与肝癌的发生和侵袭转移.

Expression of KLF8 in hepatocellular carcinoma and its significance

Objective To investigate the expression and role of Kruppel-like factor 8 (KLF8) in hepatocellular carcinoma (HCC).Methods The KLF8 mRNA and protein levels in different HCC cell lines and HCC tissues were determined by RT-PCR, immunochemistry and Western blot respectively.Resuits Metastatic potential HCC cell lines had higher KLF8 mRNA and protein levels than primary HCC cell lines (P <0.05).Relative KLF8 mRNA levels in normal liver tissues (6 cases) ,HCC tissues (17 cases), and recurrent HCC tissues (6 cases) were 0.2377 ± 0.0658, 0.4683 ± 0.2073, and 0.6669 ± 0.0239, respectively (P < 0.05).Metastasis specimens exhibited the highest level of KLF8 expression (5/6,83.3 % strong positive), and primary tumor tissue specimens (27/33,81.8%) had higher level of KLF8 expression than normal (1/6,16.7%) and liver cirrhosis tissue specimens (2/6,33.3%), (P < 0.01).Conclusion Abnormally activated KLF8 expression may contribute to HCC development and progression.