脑外伤大鼠脑内bFGF mRNA和TNF蛋白的表达

目的　研究脑外伤大鼠脑内碱性成纤维生长因子(bFGF)mRNA和肿瘤坏死因子(TNF)蛋白表达的时程变化,探讨脑外伤后神经功能修复的内源性机制。方法　自由落体法制作脑外伤大鼠模型,用Northernblot和吸光度扫描分析bFGFmRNA表达,Westernblot检测TNF蛋白表达。结果　脑外伤大鼠脑内bFGFmRNA和TNF蛋白的表达在12～2 4h开始增高,3d时达到高峰,7d时逐渐减弱。结论　脑外伤后,随着大鼠脑内损伤因子TNF蛋白表达的增强,保护因子bFGFmRNA的表达也相应增强,二者的相互作用这可能是脑外伤后神经功能损伤与修复的主要机制之一。

Expressionsin of bFGF mRNA and TNF protein in rats brain following brain trauma

Objective To investigate bFGF mRNA expression and of TNF protein expression after brain trauma in rats brain and providing the evidence of rehabilitation of neural function. Methods To set up brain trauma model by weight-dropping. Expression of bFGF and TNF were analyzed by Northernblot, Westernblot and optical density scanning. Results bFGF mRNA expression and TNF protein expression were peaked in the 3th day, and decreased on the 7th day detected. Conclusion TNF protein expression increased with corresponding enhancement of bFGF expression, a kind of protective factor in rats brain following brain trauma. Probably this is noe of the endogenous mechanisms for rehabilitation of neural function in the course of brain trauma.