银杏叶提取物防治兔心肌缺血再灌注损伤实验研究

目的　探讨家兔心肌缺血再灌注损伤中血小板活化因子 (Platelet-activatingfactor,PAF)与血小板活化因子 -乙酰水解酶(PAF -Acetyl-hydrolase,PAF -AH)的变化以及银杏叶提取物 (ExtraGinkgoBiloba ,EGB)对家兔心肌缺血再灌注损伤的防治效果。方法　取 30只家兔随机分为 3组 :正常家兔组 (n =1 0 ) ;缺血再灌注对照组 (n =1 0 ) ;EGB治疗缺血再灌注组 (n =1 0 ) ,按文献建立模型 ,检测各组PAF、PAF -AH、丙二醛 (MDA)和ATP的变化。结果　缺血再灌注对照组与EGB治疗缺血再灌注组PAF含量显著高于正常家兔组 (P <0 .0 1 ) ,而PAF -AH的活性显著低于正常家兔组 (P <0 .0 1 ) ;缺血再灌注对照组心肌组织MDA含量显著高于EGB预处理组 (P <0 .0 1 ) ,而ATP的含量显著低于EGB预处理组 (P <0 .0 1 )。结论　PAF与AF -AH的变化在心肌缺血再灌注损伤中发挥重要作用 ,EGB对心肌缺血再灌注损伤有一定的防治作用 ,其机制与EGB阻断PAF -R等因素相关。

EXPERIMENTAL STUDY OF THE PREVENTIVE AND THERAPEUTIC EFFECT OF EXTRA GINKGO BIOL BA ON INJURY CAUSED BY MYOCARDIAL ISCHEMIA REPERFUSION IN RABBITS

Objective To study the roles of plasma PAF and PAF-AH and the preventive and therapeutic effects of Extra Ginkgo Biolba (EGB) on the injury caused by myocardial ischemia reperfusion in rabbits. Methods 30 male rabbits were randomly divided into 3 groups: normal group (n=10); the model myocardial ischemia reperfusion control group (n=10); EGB treatment group (n=10). Models were set up according to literature, and the changes of PAF,PAF-AH, MDA and ATP in the respective groups were tested. Results The concentration of PAF in the control group and EGB group was higher ( and the activation of PAF-AH was lower) than that in the normal group with significant statistical difference (P<0.01). The concentration of cardiac muscle MDP in the control group was higher ( and the concentration of cardiac muscle ATP was lower) than that in EGB group again with significant statistical difference (P<0.01). Conclusion The data indicate that the changes of PAF and PAF-AH are related to the occurrence and development in the injury of myocardial ischemia reperfusion; EGB has preventive and therapeutic effects in injury of myocardial ischemia reperfusion.