自体移植皮片中黑皮素1受体表达与黑色素含量的相关性研究

目的检测黑皮素1受体(melanocortin 1 receptor,MC-1R)及黑色素在不同类型自体移植皮片中的表达,并与自身正常皮肤作对照,初步认识MC-1R在自体移植皮片过度色素沉着中的作用。方法取颈部自体皮片移植术后1年的皮片(包括全厚皮片、中厚皮片及刃厚皮片),以及原供区、受区周围皮肤作为正常对照,采用免疫组织化学方法、银染-丽春红法检测自体移植皮片与正常皮肤中MC-1R表达及黑色素含量。结果MC-1R的表达定位于表皮基底部黑色素细胞、角质形成细胞胞浆;在各类自体移植皮片中均呈阳性表达,皮片愈薄MC-1R表达愈明显;MC-1R在各类移植皮片中的表达与两类正常对照皮肤比较差异均有统计学意义(P<0.01),但两类正常对照皮肤内的表达差异无统计学意义(P>0.01)。不同类型移植皮片表皮中黑色素含量较两种正常对照皮肤均明显增加,且差异有统计学意义(P<0.01);且不同类型移植皮片表皮中黑色素含量的差异亦均有统计学意义(P<0.01),皮片愈薄,黑色素含量愈多;皮片中黑色素含量与MC-1R表达量呈正相关。结论自体移植皮片中黑色素含量与MC-1R表达量呈正相关,MC-1R在自体移植皮片中的高表达能使皮片中黑色素含量增加,在自体移植皮片过度色素沉着中起重要调控作用。

CORRELATION OF THE EXPRESSION OF MELANOCORTIN 1 RECEPTOR AND MELANIN CONTENTS IN HUMAN SKIN AUTOGRAFTS

Objective To detect the expression of melanocortin 1 receptor (MC-1R) and the melanin contents in human skin autografts and the normal skin, to elucide the role of MC-1R in hyperpigmented process of skin autografts. Methods Skin autografts and normal skin samples were obtained from skin graft on neck who need reoperation to release contractures after 1 year of operations. Immunohistochemical technique was performed to detect the expression and distribution of MC-1R in skin autografts(include full thickenss skin autografts, medium thickness skin autografts, and razor-thin skin autografts) and normal skin respectively. Masson-Fontana staining technique was performed to detect the melanin contents in all sorts specimens respectively. Results The expression of MC-1R was located on cell membrane and cytoplasm of melanocyte and keratinocyte in epidermal. The expression of MC-1R in most skin autografts was much stronger than that of control normal skins; the thinner skin autografts were, the more obvious expressions of MC-1R in skin autografts were. The expressions of MC-1R in all sorts of skin autografts were of significant differences compared with that in normal skins(P<0.01); the expression of MC-1R in normal skin of donor area was no significant differences compared with normal skin around recipient area(P>0.01). The contents of melanin in skin autografts were increased obviously and there were significant differences compared with that in normal skins(P<0.01); the contents of melanin among all sorts of skin autografts were of significant differences (P<0.01). The thinner skin autografts were, the more melanin contents in skin autografts. The expression of MC-1R was positively correlated with the contents of melanin in epidermis. Conclusion The expression of MC-1R in skin autografts is significantly higher than that in normal skin and is correlated positively with the contents of melanin in skin autografts. Overexpression of MC-1R may play an important role in hyperpigmented process of skin autografts.