| 酸性染料比色法测定白芥子中总生物碱的含量 |
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| 引用本文： | 吕伟,田实民.酸性染料比色法测定白芥子中总生物碱的含量[J].中药研究与信息,2011(4):18-20. |
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| 作者姓名： | 吕伟 田实民 |
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| 作者单位： | 枣庄市药品检验所,山东枣庄277102 |
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| 摘 要： | 目的-建立白芥子中总生物碱含量测定的方法。方法：采用酸性染料比色法测定白芥子中总生物碱含量。结果：酸性染料比色法显色的条件为pH4．2的缓冲液10mL，0．04％的溴甲酚绿溶液2．0mL，三氯甲烷萃取4次，每次5mL，检测波长332nm。芥子碱硫氰酸盐在1．696～16．960μg·mL。与吸光度呈良好的线性关系，平均回收率99．75％，RSD=1．16％（n=6）。结论：该方法灵敏度高，准确性，重现性好。
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| 关 键 词： | 酸性染料比色法 白芥子 总生物碱 含量测定 |
Determination of the Total Alkaloids in Semen Sinapis Alba by Acid Dye Colorimetry |
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| LU Wei,TIAN Shi-min.Determination of the Total Alkaloids in Semen Sinapis Alba by Acid Dye Colorimetry[J].Research & Information on Traditional Chinese Medicine,2011(4):18-20. |
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| Authors: | LU Wei TIAN Shi-min |
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| Institution: | 1. Zaozhuang Institute of Drug Control, Zaozhuang 277102, China) |
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| Abstract: | Objective：To investigate the method of determination of total alkaloids in Semen Sinapis alba. Methods ： The Contents of total alkaloids were determined by Acid dye colorimetry. Results： Acid dye color conditions ： pH 4. 2 buffer 10 mL,0.04 % bromocresol green liquid 2. 0 mL,chloroform extraction four times,each time use 5 mL, and the wavelength was at 332 nm. The linear range of Sinapine thiocyanate was 1. 696 - 16. 960 μg·mL^-1 ,the mean recovery was 99.75 % ,RSD = 1.16 % （n = 6）. Conclusion：This method is simple,sensitive and accurate with good reproducibility. |
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| Keywords: | Acid Dye Colorimetry Semen Sinapis alba Total Alkaloids Determination |
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