HPLC测定血栓心脉宁片中芦丁和丹酚酸B的含量

目的：建立HPLC同时测定血栓心脉宁片中芦丁与丹酚酸B含量的方法。方法：选用AgilentHC—C18（4．6mm×150mm，5μm）色谱柱，流动相为甲醇-乙腈-0．5％甲酸（30：9：61），流速为0．7mL·min^-1，检测波长为257nm。结果：丹酚酸B在0．207—2．07斗g与峰面积呈良好线性（r=0．9996），平均回收率为98．6％，RSD=1．0％（n=6）；芦丁在0．2028—2．028μg与峰面积呈良好线性（r=0．9999），平均回收率为99．5％，RSD=0．5％（n=6）。结论：该方法简便、准确、重复性好，可用于血栓心脉宁片中芦丁与丹酚酸B含量测定，以控制该制剂的质量。

HPLC Determination of Rutin and Salvianolic acid B in Xueshuan Xinmaining Tablets

Objective：To establish an new assay method for rutin and Salvianolic acid B in xueshuan xinmaining tablets by HPLC. Methods： The AgilentHC-C18 column （4. 6 mm × 150 mm,5 μm） was adopted with a mobile phase of Methanol-acetonitrile -0. 5% formic acid（30： 9：61 ）, the flow rate was 0. 7 mL · min^-1 , the wavelength of detection was set at 257 rim. Results： Salvianolic acid B＇s linearity was obtained within the range of 0. 207 -2. 07 txg （r =0. 999 6）, and the average recovery（n = 6） was 98.6 %, and RSD was 1.0 %. Rutin＇s linearity was obtained within the range of 0.2028 -2.028μg （r =0.999 9）, and the average recovery（n =6） was 99.5 %, and RSD was 0.5 %. Conclusion： The method is simple, reliable and reproducible, and can be used to control the quality of xueshuanxinmaining tablets.