| 不同pH值和酶提取体系对丹参抗氧化酶活性的影响 |
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| 引用本文： | 李璇,王升,岳红,林淑芳,吴志刚,郭兰萍. 不同pH值和酶提取体系对丹参抗氧化酶活性的影响[J]. 中药研究与信息, 2011, 0(12): 46-49 |
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| 作者姓名： | 李璇 王升 岳红 林淑芳 吴志刚 郭兰萍 |
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| 作者单位： | [1]西南交通大学生命科学与工程学院,四川成都610031 [2]中国中医科学院中药研究所,北京100700 [3]北京中医药大学中药学院,北京100102 |
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| 基金项目： | 国家自然科学基金（81130070,81072989）,国家中医药管理局行业科研专项（201107009）,国家科技重大专项（2009ZX09502-026,2009ZX09301-005）;中国中医科学院课题ZZ20090302;发改办高技【2010】1196. |
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| 摘 要： | 目的：考察不同的pH值磷酸缓冲溶液，以及加入EDTA—Na2或／和PVP的酶提取体系对丹参抗氧化酶活性的影响。方法：使用不同pH值的磷酸缓冲溶液考察了丹参SOD、CAT、POD酶活性在冷冻前后10d的差异；在最优的pH值磷酸缓冲溶液中加入不同物质考察SOD、CAT、POD的活性差异。结果：使用0．05mol·L^-1，pH值为7．0的磷酸缓冲溶液，内含0．1mmol·L^-1 EDTA—Na2，1％（w／v）聚乙烯毗咯烷酮（PVP）作为提取体系，检测SOD酶、CAT酶和POD酶活性获得的数据较优。
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| 关 键 词： | 酶提取体系 pH值 抗氧化酶 |
Effect of Different pH and Enzyme Extraction System on the Activity of the Antioxidant Enzymes System in Salvia miltiorrhiza |
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| Li Xuan,Wang Sheng,Yue Hong,Lin Shufang,Wu Zhigang,Guo Lanping. Effect of Different pH and Enzyme Extraction System on the Activity of the Antioxidant Enzymes System in Salvia miltiorrhiza[J]. Research & Information on Traditional Chinese Medicine, 2011, 0(12): 46-49 |
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| Authors: | Li Xuan Wang Sheng Yue Hong Lin Shufang Wu Zhigang Guo Lanping |
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| Affiliation: | 1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China; 2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Bering 100700, China; 3. School of Chinese Material Medica, Beijing University of Chinese Medicine, Beijing 100029 ,China) |
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| Abstract: | Objective：Investigate phosphate buffer solution （PBS）with different pH and enzyme extraction system on the activity of the antioxidant enzymes system in Salvia miltiorrhiza. Methods： Examine the activities of SOD, CAT and POD which are extracted by PBS with different pH before and after storing in freezer for 10 days. Add EDTA- Na2 and polyvinylpyrrolidone （PVP） in the PBS with optimal pH to investigate the activities of SOD, CAT and POD. Results：PBS with pH 7. 0 which contains 0. 1 mmol·L^-1 EDTA-Na2 and 1% PVP is the optimal extraction system. |
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| Keywords: | Enzymes extraction system pH Antioxidant enzymes system |
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