丹参有效成分提取的研究

目的：确定丹参药材的最佳提取工艺。方法：以丹参酮ⅡA和丹酚酸B的含量转移率为综合考察指标，用正交设计试验对丹参提取工艺条件进行优选。结果：丹参中脂溶性成分的最佳提取工艺为将丹参饮片用10倍量95％乙醇提取2h，提取液在40℃浓缩至规定量；丹参中水溶性成分的最佳提取工艺为用8倍量50％乙醇提取3次，第1次2h，其余2次各1h，将3次提取液在40℃浓缩至规定量；对两种工艺进行优化，结合实际生产得出最佳提取工艺为先用95％乙醇8倍量提取2h，残渣加8倍量50％乙醇提取2次，第1次2h，第2次1h，提取液分别在40℃浓缩至规定密度。结论：此工艺不仅能够充分提取丹参中的有效成分，减少在提取过程中有效成分的破坏，而且可以更好地发挥药物疗效，保证质量。

Study on Extraction Active Ingredients of Salvia Miltiorrhiza Bge.

Objective： To determine the best extraction process for Salvia miltiorrhiza Bge.. Methods： The content shift rates of tanshinone IIA and salvianolic acid B, as the evaluating indictors in orthogonal experimental design, were applied to value the process. with 10 times 95 % ethanol for 2 hours, and the extraction was enriched at 40 ℃ ; the optimum extraction process for water-soluble ingredients was with 8 times 50 % ethanol for three times, 2 hours for the first, and 1 hour for the second and third respectively, then the solution was concentrated to the requirement at 40 ℃. According to the need of manufacture, the optimum extraction process was determined with 8 times 95 % ethanol for 2 hours, and, the residue was with 8 times 50 % ethanol for two times with 2 and 1 hour respectively. The extractions were concentrated at 40 ℃. Conclusion： The active ingredients were extracted fully with this process and were not damaged, for assure quality.