HPLC测定防风中色原酮的含量

目的：建立高效液相法测定防风中4种色原酮的方法。方法：采用Diamonsil C18（4．6mm×200mm，5Ixm）色谱柱；流动相：甲醇-水梯度洗脱（甲醇：0～15min，20％～45％；15～25min，45％～70％）；流速1mL．min^-1。柱温30℃；波长254nm。结果：升麻素苷在0．15—3μg、升麻素在0．02～0．4μg、5-O-甲基维斯阿米醇苷在0．25—5μg、亥茅酚苷在0．01～0．2μg线性关系良好。结论：该方法快速简便，准确可靠，可用于防风中4种色原酮的测定。

Determination of the Chromones Contents of Saposhnikovia divaricata by HPLC

Objectiv kovia divaricata. Methods： e： A HPLC method A Diamonsil C18（4. was established for determination of the chromones contents of Saposhni- 6 mm × 200 mm, 5 μm） was used with gradient elution of methanol and water that methanol increased from 20 % to 45 % in 15 minute, and then increased from 45 % to 70 % until 25 minute as mobile phase ; the flow rate was 1.0 mL · min^- 1 at 30℃ and the detector wave length was 254 nm. Results： The relationships between the concentration and the peak area of 4 chromones were good linear relation. The calibration curve of prim-O-glucosylcimifugin, cimifugin, 5-O-methylvisammioside and sec-O-glucosylhamaudol were at the range of 0.15 - 3 μg, 0.02 - 0.4 μg, 0.25 - 5μg and 0.01 - 0.2 μg. Conclusion： The method was rapid, sensitive and accurate, and could be used for the determination of chromones of S. divaricata.