改性纳米羟基磷灰石表面性质及对成骨细胞贴壁生长的影响

背景：生物材料表面性质的改变能够影响其与细胞的相互作用,并进一步影响细胞黏附能力、基因表达等行为。目的：评价改性纳米羟基磷灰石的表面性质及其对成骨细胞在材料表面黏附能力的影响。方法：参考作者既往实验制备纳米羟基磷灰石和改性纳米羟基磷灰石,利用zeta电位仪测定改性纳米羟基磷灰石的zeta电位,通过测定水在改性羟基磷灰石表面的接触角,结合成骨细胞黏附试验,分析表面改性对材料的细胞黏附能力和生物相容性的影响。结果与结论：改性纳米羟基磷灰石表面呈疏水性,接触角为93°,成骨细胞黏附实验表明,改性纳米羟基磷灰石表面比纳米羟基磷灰石表面黏附更多的成骨细胞,表明表面改性增强了纳米羟基磷灰石的生物相容性。

Effect of surface properties of modified hydroxyapatite on the adherent growth of osteoblasts

BACKGROUND： The alteration of biomaterial surface properties may affect interaction between the biomaterials and cells, which will elicit diverse cell behaviors, such as cell adhesion, gene expression and so on. OBJECTIVE： To investigate the effects of surface properties of a new type of polymer modified hydroxyapatite （HA） and to determine its effects on adhesion ability of osteoblasts. METHODS： HA and modified HA were prepared according to author’s previous experiments. The zeta potential of modified HA was measured by using zeta potential analyzer. The water contact angle and cell attachment test were carried out to investigate the effect of surface modification on the cell adhesion ability and the biocompatibility of modified HA. RESULTS AND CONCLUSION： The surface of modified HA was hydrophobic, and its water contact angle was 93°. Cell attachment test showed that the number of osteoblasts attached on the modified HA surface was higher than that on the HA surface. It is indicated that the surface modification enhances the biocompatibility of HA.