胰岛素对溺水后幼年大鼠应激性高血糖的疗效观察

Objective To explore the alteration of insulin signal transduction in drowning-induced stress hyperglycemia and to investigate therapeutic effect of insulin on it.Methods Stress hyperglycemia model was induced by drowning.Thirty-two infant rats were randomized into control,drowning,air resuscitation and air-insulin resuscitation groups.Insulin was injected abdominally into the air-insulin resuscitation rats.Fasting serum glucose and insulin were determined routinely,and protein expressions and serine phosphorylation levels of insulin receptor substrate 1(IRS-1) in muscle tissue were detected by Western blot and immunoprecipitation.The expressions of glucose transporter 4(GLUT4) in the intracellular and plasma membranes of muscle tissue were detected by Western blot.Results In the drowning group,insulin resistance index(HOMA-IR) increased significantly as compared with that of the control group,but the level of IRS-1 had no significantly change as compared with the other three groups.As compared with that of the drowning group(0.71±0.12),IRS-1 serine phosphorylation levels of the two resuscitation groups decreased significantly(0.56±0.13 and 0.46±0.08 respectively).The intracellular GLUT4 expression in the two resuscitation groups(1.82±0.11 and 1.96±0.28 respectively)increased significantly in contrast to that of the drowning group(1.45±0.15),and the air-insulin resuscitation groups showed an especially high increase of intracellular GLUT4 expression.Conclusion During the drowning-induced stress hyperglycemia,the alteration of serine phosphorylation and GLUT4 distribution is one of the important mechanism of insulin resistance.Insulin may decrease the blood glucose through decreasing serine phosphorylation levels of IRS-1 and increasing the intracellular GLUT4 expressions.

Effects of insulin on stress hyperglycemia in drowning infant rats.

Objective To investigate the therapeutic effects and mechanism of insulin on stress hy-perglycemia in the drowning rats. Methods Stress hyperglycemia rat model was induced by drowning. Thir-ty-two rats were randomized into control, drowning, air resuscitation and air-insulin resuscitation groups. Insu-lin was injected into the air-insulin resuscitation rats via abdomen. The glucose,insulin, TNF-α level of serum were determined during the drowning and resuscitation. 3H-D-glucose was used to measure the glucose uptake rate (GUR) of skeletal muscles. Results Compared with those in the control group, the GUR decreased,in-sulin resistance index (HOMA-IR) increased significantly in drowning group. Compared with those in the air resuscitation group, the blood glucose and TNF-α were decreased, the GUR increased significantly in air-insu-lin resuscitation group. Conclusion Insulin therapy may decrease the blood glucose and TNF-α level, in-crease the GUR of stress hyperglycemia,and the mortality of drowning rats can be decreased.