不同鼠疫检测技术在河北省鼠疫监测中的应用研究

目的通过用鼠疫血凝试验、胶体金标记免疫层析(金标)和ELISA 3种方法检测啮齿动物血清,用金标、ELISA、PCR与鼠疫反向血凝试验和细菌分离检测啮齿动物脏器,评价其在鼠疫监测中的应用效果。方法用鼠疫血凝试验、金标和ELISA 3种方法检测啮齿动物血清;用金标、ELISA、PCR、鼠疫反向血凝试验和细菌分离检测啮齿动物脏器。结果鼠疫血凝试验、金标和ELISA 3种方法对啮齿动物血清的阳性检出率分别为0.32%、0.32%和1.26%。金标、ELISA、PCR、鼠疫反向血凝试验和细菌分离对啮齿动物脏器的阳性检出率分别为3.15%、0.70%、4.90%、0.70%和0.70%。结论金标、ELISA、PCR值得推广应用,非常适合基层和快速诊断应用。

Study on application with different testing technologies of plague surveillance in Hebei province

Objective To value application effects on plague surveillance using different methods.Methods Rodent serums were detected by indirect hemagglutination assay(IHA),gold-immunochromatography assay(GICA-Ag),enzymelinked immunosorbent assay(ELISA-Ag).Rodent viscera were detected by gold-immunochromatography assay(GICA-Ab),enzymelinked immunosorbent assay(ELISA-Ab),polymerase chain reaction(PCR),bacterial isolation,reverse indirect hemagglutination assay(RIHA).Results Antibody detection rates in serums using IHA,GICA-Ag, ELISA-Ag were 0.32%,0.32%,1.26%,respectively.Positive detection rates of GICA-Ab,ELISA-Ab,PCR,RIHA,bacterial isolation in rodent viscera were 3.15%,0.70%,4.90%,0.70%,0.70%,respectively.Conclusion GICA,ELISA and PCR are valueable to extend and apply.They are suitable to use in basic level and fast diagnosis.