血清白细胞介素12及肿瘤坏死因子在病毒性肝炎发病过程中的作用研究

目的探讨IL-12与TNF在病毒性肝炎发病过程中的变化及临床意义。方法双抗体夹心酶联免疫吸附法(ELISA)检测64例病毒性肝炎患者及20例正常对照血清IL-12与TNF水平。结果病毒性肝炎患者血清IL-12与TNF水平明显高于正常对照,与临床病型关系密切,差异有显著性意义(P<0.01)。恢复期,血清IL-12水平正常(P>0.05);血清TNF在急性肝炎正常(P>0.05),慢性肝炎及部分重型肝炎仍高于正常(P<0.05)。血清IL-12与TNF水平与血清总胆红素及ALT水平呈正相关。结论IL-12及TNF参与了病毒性肝炎的免疫病理过程。早期检测血清IL-12与TNF水平,可判断病情的严重程度及预后,指导临床诊断及治疗。

Role of serum interleukin - 12 and tumor necrosis factor on human viral hepatitis

Objective To study the clinical significance of serum interleukin-12(IL-12) and Tumor necrosis factor(TNF) in patients with viral hepatitis.Methods The leve of IL-12 and TNF mas measured by enzyme linked immunosorbent assay(ELISA) in 64 patients with viral hepatitis and 20 healthy adults subject as controls.Results The level of IL-12 and TNF was significantly higher in patients with viral hepatitis than those in healthy controls.The variations of the serum IL-12 and TNF was closely associated with the clinical types of viral iepatitis(P<0.01).In convalescence.The serum IL-12 leve was normal(P>0.05),The serum TNF level was normal in AH(P>0.05) and was higher than normal in CH and in 4 cases of SH(P<0.05).The serum IL-12 and TNF leve was positively correlated with serum TBILI and ALT levels.Conclusions IL-12 and TNF participates in the activity process of viral hepatitis,to measure the levels of serum IL-12 and TNF in early stage may reflect the severity of viral hepatitis and be helpful to prognosticating the disease and guiding in clinical diagnosis and treatment.