增强型绿色荧光蛋白基因对树突状细胞功能的研究

目的研究瘤内注射的树突状细胞（DC）的迁移及在肿瘤局部的作用。方法建立大鼠C6脑胶质瘤颅内肿瘤模型，脂质体介导的增强型绿色荧光蛋白（EGFP）基因转染DC细胞，瘤内注射DC后取大鼠同侧的淋巴结在荧光显微镜下检测，并用流式细胞仪检测肿瘤周围组织CD4^＋T和CD8^＋T细胞的浸润。结果同侧的颈深部淋巴结可见EGFP表达的DC，对侧淋巴结未见到。DC治疗组证实有CD4^＋T和CD8^＋T明显浸润（CD4^＋T8．19％，CD8^＋T11．05％），RPMI1640治疗组CD4^＋T0．95％和CD8^＋T1．17％，生理盐水对照组CD4^＋T0．62％和CD8^＋T0．77％。结论瘤内注射的DC不仅能够迁移，而且有效地引起肿瘤局部的T细胞显著浸润，为瘤内注射DC细胞提供了依据。

Effect of enhanced green fluorescent protein on the function of dendritic cells

Objective To study whether intracranially inoculated dentritic cells (DC) could migrate and the local antitumor cytoxicity. Methods Intratumoral C6 brains glioma models of rats were made. After transfection by lipofectamine with enhanced green fluorescent protein (EGFP), DCs were inoculated direcdy into the tumor bed, and deep cervical lymph nodes dissected out and visualized under a fluorescence microscope.Flow cytometry was performed to analyze T-cell invasion around the tumor. Results Deep cervical lymph nodes ipsilateral to the site of implantation were infiltrated with numerous GFP expressing cells. In contrast, contralateral lymph nodes were not shown. DC treated tumors demonstrated robust infiltration with CD4+ and CD8+ T cells (CD4+ T cells 8.19 %, CD8+ T cells 11.05 % ), RPMI 1640 (CD4+ T cells 0.95%, CD8+ T celsl 1.17%), normal saline (CD4+ T cells 0.62% and CD8+ T cells 0.77 %). Conclusion Intracranially administered DC can not only migrate, but also result in significant T cell infiltration to tumor effectively.