| Dot—ELISA早期鉴别阳性钉螺的实验研究 |
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| 引用本文： | 楼涤 娄文娴. Dot—ELISA早期鉴别阳性钉螺的实验研究[J]. 中国血吸虫病防治杂志, 1998, 10(2): 83-85 |
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| 作者姓名： | 楼涤 娄文娴 |
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| 作者单位： | 浙江省医学科学院寄生虫病研究所!杭州，310013，浙江省医学科学院寄生虫病研究所!杭州，310013，浙江省医学科学院寄生虫病研究所!杭州，310013，上海第二医科大学寄生虫学教研室 |
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| 基金项目： | 卫生部与TDR联合科研管理委员会（JRMC）资助 |
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| 摘 要： | 应用抗血吸虫单克隆抗体的Dot－ELISA检测早期感染钉螺体内血吸虫抗原，并以镜检为对照。钉螺感染后，逐周取样30只进行检测，结果显示感染3wk后，钉螺血吸虫抗原的阳性率为70％。以后逐周增加，5wk为83．33％，8wk为96．67％。提示该法似有早期鉴别阳性钉螺的效能。
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| 关 键 词： | 单克隆抗体 钉螺 日本血吸虫 Dot－ELISA |
EXPERIMENTAL STUDIES ON THE EARLY-STAGE IDENTIFICATION OF POSITIVE INFESTED SNAILS BY DOT-ELISA |
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| Lou Di, Zheng Zhiguo,Shen Bingrong, Lou Wenxian. EXPERIMENTAL STUDIES ON THE EARLY-STAGE IDENTIFICATION OF POSITIVE INFESTED SNAILS BY DOT-ELISA[J]. Chinese journal of schistosomiasis control, 1998, 10(2): 83-85 |
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| Authors: | Lou Di Zheng Zhiguo Shen Bingrong Lou Wenxian |
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| Abstract: | The Dot-ELISA was established by monoclonal antibody to detect schistosome antigen in snails,and the comparison with microscopical examination was observed. 30 snails exposed to miracidia of S. japonicum in mass were detected individually every week. The positive rate of schistosome antigen in snails was 70% in 3 weeks post-exposure,and then increased later to 83. 33 % in 5 weeks and 96.67% in 8 weeks post-exposure. The result suggested that the method is protensire for identifvation of positive snalls in early-stage. |
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| Keywords: | monoclonal antibody snail schistosoma japonicum Dot-ELISA |
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