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| 姜黄素对Caco-2细胞上P-糖蛋白活力及MDR1 mRNA表达的影响 | |
| 引用本文： | 唐靖,宋娟,彭文兴.姜黄素对Caco-2细胞上P-糖蛋白活力及MDR1 mRNA表达的影响[J].中南药学,2011,9(12):889-893. |
| 作者姓名： | 唐靖 宋娟 彭文兴 |
| 作者单位： | 1. 长沙医学院,长沙410219;中南大学湘雅二医院,长沙410011 2. 中国医药保健品有限公司,北京,100061 3. 中南大学湘雅二医院,长沙,410011 |
| 摘 要： | 目的研究姜黄素对Caco-2细胞上P-糖蛋白(P-gp)功能和表达的影响,并从分子水平研究其对MDR1mRNA表达的影响。方法采用流式细胞仪测定P-gp底物——罗丹明-123在细胞中的浓度,并分析Caco-2细胞上P-糖蛋白的表达量;采用RT-PCR法分析Caco-2细胞上MDR1基因mRNA的表达量。结果低、中、高浓度(0.1、0.5、1.0μmol.L-1)的姜黄素增加了罗丹明-123的外排,其外排量分别增加了6.2%、22.2%和33.8%(P<0.05);0.1～1.0μmol.L-1姜黄素与细胞孵育72h后,细胞膜上的荧光强度随浓度增加而增强(P<0.05),P-gp表达量是空白对照组的4倍,MDR1基因mRNA表达水平上调了1.58倍。结论姜黄素并不是P-gp的抑制剂,它能在较低浓度以及较短时间内(1h)增强Caco-2细胞上P-gp的功能活性,从而减少罗丹明-123在细胞内的蓄积。长时间(72h)应用姜黄素可以诱导P-gp和MDR1mRNA的表达。姜黄素将有可能引起相应的药物-药物/食物的相互作用。 |
| 关 键 词： | 姜黄素 P-糖蛋白 MDR1 mRNA |
Effect of curcumin on P-glycoprotein activity and expression of MDR1 gene mRNA in Caco-2 cells |
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| TANG Jing , SONG Juan , PENG Wen-xing.Effect of curcumin on P-glycoprotein activity and expression of MDR1 gene mRNA in Caco-2 cells[J].Central South Pharmacy,2011,9(12):889-893. | |
| Authors: | TANG Jing SONG Juan PENG Wen-xing |
| Institution: | TANG Jing1,2,SONG Juan3,PENG Wen-xing2(1.Changsha Medical College,Changsha 410219,2.Second Xiangya Hospital,Central South University,Changsha 410011,3.CNTIC TRADING Co.,Ltd,Beijing 100061) |
| Abstract: | Objective To determine the effect of curcumin on the activity of P-glycoprotein(P-gp) and expression of MDR1 mRNA in Caco-2 cells. Methods Flowcytometry was used to determine the effect of curcumin on the efflux of Rhodamine-123 and the expression of P-gp in Caco-2 cells.RT-PCR was used to measure the expression level of MDR1 gene mRNA in Caco-2 cell. Results The efflux quantity of Rhodamine-123 was increased by 6.2%,22.2%,and 33.8%(P<0.05),compared with that of controls when being treated with curcumin at ... |
| Keywords: | curcumin P-glycoprotein MDR1 mRNA |
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