| HPLC测定养阴清肺颗粒中哈巴俄苷的含量 |
| |
| 引用本文： | 陈华国,周欣,赵超,龚小见. HPLC测定养阴清肺颗粒中哈巴俄苷的含量[J]. 中国实验方剂学杂志, 2010, 16(7): 44-46 |
| |
| 作者姓名： | 陈华国 周欣 赵超 龚小见 |
| |
| 作者单位： | 1. 贵州师范大学,天然药物质量控制研究中心,贵阳,550001
2. 贵州师范大学,天然药物质量控制研究中心,贵阳,550001;贵州省山地环境信息系统与生态环境保护重点实验室,贵阳,550001 |
| |
| 基金项目： | 贵州省中小企业局平台建设项目，贵州省发展和改革委员会 |
| |
| 摘 要： | 目的:建立养阴清肺颗粒中哈巴俄苷的测定方法。方法:采用反相高效液相色谱法,HypersilODS(4.6mm×250mm,5μm)柱,流动相为乙腈和1%醋酸水溶液,线性梯度洗脱(15∶85～60∶40)(0～25min),流速1mL·min-1,检测波长278nm,柱温25℃。结果:哈巴俄苷浓度在0.61～12.21μg线性关系良好,r=0.9999,平均回收率为97.02%,RSD为0.6%(n=9)。结论:该方法分离效果好,简便准确,可用于养阴清肺颗粒中哈巴俄苷的含量测定,以控制其质量。
|
| 关 键 词： | 养阴清肺颗粒 哈巴俄苷 高效液相色谱法 含量测定 |
| 收稿时间： | 2009-12-08 |
Determination of Harpagoside in Granules of Yangyin Qinfei by RP-HPLC |
| |
| CHEN Hua-guo,ZHOU Xin,ZHAO Chao and Gong Xiao-jian. Determination of Harpagoside in Granules of Yangyin Qinfei by RP-HPLC[J]. China Journal of Experimental Traditional Medical Formulae, 2010, 16(7): 44-46 |
| |
| Authors: | CHEN Hua-guo ZHOU Xin ZHAO Chao Gong Xiao-jian |
| |
| Affiliation: | Research Center for Quality Control of Natural Medicine,Guizhou Normal University, Guiyang 550001,China; Key laboratory for Information System of Mountainous Areas and Protection of Ecological Environment,Guiyang 550001,China;Research Center for Quality Control of Natural Medicine,Guizhou Normal University, Guiyang 550001,China; Key laboratory for Information System of Mountainous Areas and Protection of Ecological Environment,Guiyang 550001,China;Research Center for Quality Control of Natural Medicine,Guizhou Normal University, Guiyang 550001,China; Key laboratory for Information System of Mountainous Areas and Protection of Ecological Environment,Guiyang 550001,China;Research Center for Quality Control of Natural Medicine,Guizhou Normal University, Guiyang 550001,China; Key laboratory for Information System of Mountainous Areas and Protection of Ecological Environment,Guiyang 550001,China |
| |
| Abstract: | |
| |
| Keywords: | Granules of Yangyin Qinfei harpagoside HPLC content determination |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
|
点击此处可从《中国实验方剂学杂志》下载全文 |
|
