淋球菌对头孢菌素类药物敏感性的检测

目的评估药敏纸片琼脂扩散法（K-B法）检测淋球菌对三代口服头孢菌素类药物敏感性的符合率。方法中国医学科学院皮肤病研究所性病科门诊收集的100株淋球菌临床分离菌株。采用K-B法检测淋球菌对头孢克肟、头孢泊肟、头孢布坦3种药物的敏感性；Etest法检测淋球菌对头孢克肟、头孢泊肟的敏感性；最低抑菌浓度琼脂稀释法（MIC法）检测淋球菌对头孢布坦的敏感性。结果K-B法和Etest法对头孢克肟敏感菌株的检测率分别为92．0％和100％；K-B法和Etest法对头孢泊肟敏感菌株的检测率分别为94．9％和100％；K-B法和MIC法对头孢布坦敏感菌株的检测率分别为93．0％和95．3％。结论K-B法对3种药物敏感菌株的检测率与Etest或MIC法检测结果差异无统计学意义。

Testing of cephalosporin susceptibility in Neisseria gonorrhoeae .

Objective To compare the performance of the Kirby-Bauer disk diffusion （K-B） method, E-test, and agar dilution method in the testing of cephalosporin susceptibility in Neisseria gonorrhoeae （NG）. Methods Totally, 100 NG isolates collected from outpatients in the Department of Venereology, Institute of Dermatology, Chinese Academy of Medical Sciences were included in this study. Both the K-B and E-test methods were used to evaluate the susceptibility of these strains to cefixime and cefpodoxime, and the agar dilution as well as K-B methods to determine the susceptibility to ceftibuten. Results The susceptibility rate of these strains was 92.0% and 100% to cefixime as well as 94.9% and 100% to cefpodoxime by the K-B method and E-test method respectively, 93.0% and 95.3% to ceftibuten by the K-B method and agar dilution method respectively. Conclusion The performance of K-B method is similar to that of E-test and agar dilution methods in the evaluation of susceptibility to the three cephalosporins in NG.