RP-HPLC法测定气血和胶囊中阿魏酸的含量

目的：建立高效液相色谱法测定气血和胶囊中阿魏酸含量的方法。方法：色谱柱：Ultimate XB-C18柱（250mm×4．6mm，51μm）；流动相：甲醇-0．1％磷酸（30：70），流速为1．0ml·min^-1；柱温：25℃；检测波长：320nm。结果：阿魏酸在浓度为5．0～80．9μg·ml。时线性关系良好，r=0．9999，平均回收率为101．7％；RSD为1．7％（n=6）。结论：方法简单、准确，可作为气血和胶囊的质量控制。

Determination of Ferulic acid in Qixuehe Capsules by RP-HPLC

Objective ： To establish a HPLC method for determination of Ferulic acid in Qixuehe Capsules. Method ： Ultimate XB- C18 column （ 250mm × 4.6mm, 5 μm） was used, the mobile phase consisted of methanol - 0.1% phosphoric acid solution （ 30： 70 ）, the flow rate was 1.0ml· min^-1 ;the column temperature was 25 ℃ ;the detection wavelength was 320nm. Result： The linear range of Ferulie acid was 5.0 - 80.9 μg· ml^- 1 （ r = 0. 999 9）. The average recovery was 101.7% （ RSD = 1.7%, n = 6）. Conclusion： The method is simple and accurate. It can he used for quality control of Qixuehe Capsules.