Survivin与MMP-9在子宫内膜异位症在位内膜中的表达

目的:检测子宫内膜异位症(EMs)患者在位内膜中survivin与MMP-9的表达情况并分析两者表达的相关性.方法:收集80例子宫内膜异位症患者子宫内膜标本为EMs在位内膜组(EMs组),其中临床分期Ⅰ期4例、Ⅱ期25例、Ⅲ期44例与Ⅳ期7例;增殖期47例,分泌期33例.以20例同期手术的单纯子宫肌瘤患者子宫内膜作为对照组,其中增殖期内膜7例,分泌期13例.采用免疫组化二步法检测2组标本中survivin与MMP-9的表达;并应用IPP专业图像分析系统进行定量分析.结果:survivin与MMP-9均在子宫内膜胞浆中表达.在EMs组中增殖期survivin和MMP-9及分泌期survivin表达均高于对照组(P＜0.01);其中,survivin表达与细胞周期无关,呈持续性高表达.EMs组不同临床分期间survivin表达,Ⅱ期明显高于Ⅲ期,其余各分期间表达差别无统计学意义(均P＞0.05);而MMP-9在各AFS分期中表达差别均无统计学意义(均P＞0.05).Pearson相关性分析表明survivin与MMP-9在子宫内膜异位症在位内膜中表达呈正相关(r=0.262,t=2.860,P＜0.05).结论:survivin与MMP-9在子宫内膜异位症形成及进展过程中均起关键的作用.

Expression of Survivin and MMP-9 in Eutopic Endometrium of Patients with Endometriosis

Objective:To investigate the expression of survivin and MMP-9 in the eutopic endometrium of patients with endometriosis(EMs).Methods:Eighty patients with endometriosis were selected as EMs group,four of them were in clinical stage Ⅰ,25 in stage Ⅱ,44 in stage Ⅲ and 7 in stage Ⅳ,47 in the proliferative phase and 33 in the secretory phase of the menstrual cycles.Twenty patients with hysteromyoma were selected as control group,including 7 patients in the proliferative phase and 13 in the secretory phase of the menstrual cycles.The expression levels of survivin and MMP-9 were determined by immuno-histochemical method.Image-Pro Plus 6.0(IPP),the special-purpose system for quantitative measurement of medical images,was used to analyse the results quantitatively.Results:Both survivin and MMP-9 were found positive in the plasma of the endometriosis.Both survivin and MMP-9 expression levels were higher in EMs group than those in the control group(P ＜0.01).Besides,survivin was over expressed with no relation to the menstrual cycle of the endometium.Survivin expression was higher in endometriosis stage Ⅱ than that of the stage Ⅲ(P ＞ 0.05).There was no difference in survivin expression within other stages(P ＞ 0.05).While MMP-9 expression had no difference between all the endometriosis stages(P ＞ 0.05).There was a close relationship between survivin and MMP-9 expression levels in 80 eutopic endometfium of endometriosis(r=0.262,t=2.860,P ＜ 0.05).Conclusion:The over expression of survivin and MMP-9 may play an important role in the pathogenesis of endometriosis.