| 薄层扫描法测定染料木素和大豆苷原的含量 |
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| 引用本文： | 刘燕华,陈光芝,褚新红.薄层扫描法测定染料木素和大豆苷原的含量[J].齐鲁药事,2006,25(2):98-99. |
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| 作者姓名： | 刘燕华 陈光芝 褚新红 |
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| 作者单位： | 1. 德州泰康药业有限公司,德州,253000
2. 山东三株实业有限公司,济南,250100 |
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| 摘 要： | 目的建立染料木素和大豆苷原的薄层扫描测定方法。方法薄层色谱条件是:吸附剂:聚酰胺-6薄膜,展开剂:苯-丁酮-甲醇(3∶1∶0.5),结果染料木素和大豆苷原均在250nm处有最大吸收,本法线性关系良好(染料木素:r=0.9993;大豆苷原:r=0.9995)。染料木素的平均回收率98.52%(n=5);大豆苷原的平均回收率99.45%(n=5)。结论方法简便,准确,重现性好,可用于测定染料木素和大豆苷原的含量。
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| 关 键 词： | 薄层扫描 染料木素 大豆苷原 |
| 文章编号： | 1672-7738(2006)02-0098-02 |
Determination of Genistein and Daidzein by TLCS |
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| LIU Yan-hua,CHEN Guang-zhi,CHU Xin-hong.Determination of Genistein and Daidzein by TLCS[J].qilu pharmaceutical affairs,2006,25(2):98-99. |
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| Authors: | LIU Yan-hua CHEN Guang-zhi CHU Xin-hong |
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| Abstract: | OBJECTIVE To establish a TLC scanning(TLCS)method for determination of genistein and daidzein.METHODS The conditions of the TLC were as follow:The absorber was Polyamide-6 diaphragm.The mobile phase was benzene-butanone-ethanol(3∶1∶0.5).RESULTS Genistein and daidzein had the max absorption in 250nm and the method has a good linear dependence(Genistein:r=0.9993;daidzein: r=0.9995).The average recovery was 98.52%,RSD=1.90%(genistein;n=5) and 99.45%,RSD=2.49%(daidzein,n=5).CONCLUSION The method was convenient,correct,reliable and suitable for determination of genistein and daidzein. |
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| Keywords: | TLCS genistein daidzein |
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