纤支镜肺活检材料肿瘤标记与肺癌预后的研究

目的：探讨纤支镜肺癌标本中肿瘤标记物的表达及其与预后的关系。方法：应用增生细胞核抗原（ＰＣＮＡ）、ｒａｓ－ｐ２１、ｐ５３单克隆抗体和ｃ－ｅｒｂＢ－２多克隆抗体，对７１例肺癌的纤支镜标本和１０例正常肺组织进行免疫组化染色研究。结果：１０例正常肺组织均为阴性表达。７１例肺癌中，ＰＣＮＡ、ｒａｓ－ｐ２１、ｐ５３、ｃ－ｅｒｂＢ－２抗体的阳性表达率分别为：７０．４２％；８０．２８％；５６．３４％和８１．６９％。ｃ－ｅｒｂＢ－２与肺癌分型相关，其它三者与肺癌分型、分化及ＴＮＭ分期无关。但与预后均呈负相关。结论：上述４种抗体可作为判断肺癌预后的肿瘤标记物，提示用于术前判定肺癌患者的预后是可行的。

Study on The Prognosis of Lung Carcinoma and Tumor Markers of Fiberoptic Bronchoscopic Lung Biopsy Materials

Objective:This paper was to study the relationship between the expression of tumor markers and prognosis of lung carcinoma. Methods:We studied the expression of proliferating cell nuclear antigen (PCNA), ras p21 protein, p53 protein, and c erbB 2 protein in 71 cases of fiberoptic bronchoscopic lung biopsy specimens from lung carcinoma and 10 cases from normal lung tissue by immunohistochemically. Results: The positive rate of PCNA, ras p21, p53, and c erbB 2 was 70.42%, 80.28%, 56.34%, and 81.69% in 71 cases of lung carcinoma respectively. No expression was found in normal lung tissue. The expression was all negatively related to prognosis of lung carcinoma, but did not correlate with subtype, differentiation, and TNM classification, although c erbB 2 protein expression had relation to subtype of lung carcinoma. Conclusions: The expression of PCNA, p21 protein, p53 protein, and c erbB 2 protein in bronchoscopic lung biopsy materials could be used to predict the prognosis of lung carcinoma preoperatively.