两种不同来源的EBV LMP1基因在Balb/c 3T3细胞中的表达

【目的】研究来源于鼻咽癌细胞株SUNE1及EBV标准株B95 - 8细胞中两种EBVLMP1基因在真核细胞Balb/c3T3中的表达 ,为进一步研究EBVLMP1基因的核酸疫苗打基础。【方法】将两种不同来源EBVLMP1基因的真核表达质粒 ,经脂质体法转染Balb/c 3T3细胞后 ,用Westernblot、免疫组化及PCR的方法检测不同转染细胞中EBVLMP1蛋白的表达及核酸片段。【结果】PCR结果表明在两种不同来源LMP1转染细胞中均有EBVLMP1基因序列 ,并均能表达 6 3ku的蛋白质 ,经Westernblot和免疫组化结果证实均为EBVLMP1蛋白。【结论】来源于B95 8细胞及鼻咽癌细胞SUNE1的两种EBVLMP1基因均能在真核细胞Balb/c 3T3中表达。

The Expression of EBV LMP1 Isolated from Two Different Origins in Balb/c 3T3 Cell Line

Objective To study the expression of two EBV LMP1 genes isolated from nasopharyngeal carcinoma cell line SUNE1 and EBV standard cell line B95 8 in the eukaryotic cells for further EBV LMP1 DNA immunization. Methods Transfected two diffierent origins LMP1 eukaryotic expression plasmid vector into Balb/c 3T3 cells by lipofectamin method and identified the expression of EBV LMP1 proteins in transfected cells by Western blot and immunohistochemistry. The polymerase chain reaction (PCR) was used to detect the EBV LMP1 fragment in the transfected cells. Results A 63 ku protein was observed by Western blot analysis and the positive staining of specific EBV LMP1 protein was detectable by immunohistochemical staining in both of the two LMP1 genetransfected cells. The EBV LMP1 fragment was detected in PCR products in both of these two transfected cells. Conclusion Both EBV LMP1 genes from two different origins can express in Balb/c 3T3 cell lines.