| 三七总皂甙增强人内皮源性一氧化氮合酶基因启动子活性 |
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| 引用本文： | 张丽华,贾钰华,孙学刚,邢飞跃,刘志锋,宋革,姜勇. 三七总皂甙增强人内皮源性一氧化氮合酶基因启动子活性[J]. 南方医科大学学报, 2004, 24(10): 1113-1116 |
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| 作者姓名： | 张丽华 贾钰华 孙学刚 邢飞跃 刘志锋 宋革 姜勇 |
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| 作者单位： | 1. 南方医科大学中医系,广东,广州,510515
2. 南方医科大学全军休克微循环重点实验室,广东,广州,510515 |
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| 基金项目： | 国家杰出青年科学基金(39925014)，国家自然科学基金(30030060、39800074)~~ |
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| 摘 要： | 目的探讨三七总皂甙(tPNS)对人内皮源性一氧化氮合酶(eNOS)基因启动子转录活性的调控机制。方法以基因重组技术构建人eNOS基因启动子区(-1~ -1 600 bp)驱动的萤火虫荧光素酶报告基因载体peNOS-Luc,采用脂质体介导的细胞基因共转染技术将peNOS-Luc、空载体pGL2-Basic和β-半乳糖苷酶表达质粒pCMV-β共转染NIH3T3细胞,用细菌脂多糖(LPS)、tPNS和转移生长因子β1(TGFβ1)等因子分别刺激转染后的NIH3T3细胞,检测并比较荧光素酶/β-半乳糖苷酶活性,以确定LPS、tPNS和TGFβ1对人eNOS基因启动子转录活性的影响。结果(1)酶切和测序结果均证实重组载体peNOS-Luc构建正确;(2)重组载体peNOS-Luc在NIH3T3细胞中有效表达;(3)在LPS刺激下,人eNOS启动子的转录活性降低,而在tPNS和TGFβ1刺激下,其启动子的转录活性增强,其中,TGFβ1较tPNS所致的转录活性更强。结论正确构建了人eNOS基因启动子区(-1~-1 600 bp)驱动的萤火虫荧光素酶报告基因载体peNOS-Luc;tPNS在NIH3T3细胞中增强人eNOS基因启动子的转录活性。
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| 关 键 词： | 三七总皂甙 一氧化氮合酶 基因表达调控 酶学 共转染 |
| 文章编号： | 1000-2588(2004)10-1113-04 |
| 修稿时间： | 2004-01-10 |
Effect of total Panax notoginseng saponins on the activity of human endothelial nitric oxide synthase gene promoter |
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| ZHANG Li-hua,JIA Yu-hua,SUN Xue-gang,XING Fei-yue,LIU Zhi-feng,SONG Ge,JIANG YongDepartments of Traditional Chinese Medicine and Key Laboratary of Shock and Microcirculation of PLA,Southern Medical University,Guangzhou ,China. Effect of total Panax notoginseng saponins on the activity of human endothelial nitric oxide synthase gene promoter[J]. Journal of Southern Medical University, 2004, 24(10): 1113-1116 |
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| Authors: | ZHANG Li-hua JIA Yu-hua SUN Xue-gang XING Fei-yue LIU Zhi-feng SONG Ge JIANG YongDepartments of Traditional Chinese Medicine Key Laboratary of Shock Microcirculation of PLA Southern Medical University Guangzhou China |
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| Affiliation: | ZHANG Li-hua1,JIA Yu-hua1,SUN Xue-gang1,XING Fei-yue2,LIU Zhi-feng2,SONG Ge2,JIANG Yong2Departments of Traditional Chinese Medicine1 and Key Laboratary of Shock and Microcirculation of PLA2,Southern Medical University,Guangzhou 510515,China |
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| Abstract: | |
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| Keywords: | Panax notoginseng saponin endothelial nitric oxide synthase gene expression regulation enzymologic co-transfection |
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