拟除虫菊酯对大鼠脑组织γ-氨基丁酸转氨酶活力的影响

目的　研究拟除虫菊酯对大鼠脑组织γ 氨基丁酸转氨酶 (GABAT)活力的影响。方法　应用偶联酶学紫外分光光度法 ,在体外和整体实验中 ,观察溴氰菊酯 (DM)和氯菊酯 (PM)对大鼠大脑皮层、海马、纹状体和小脑GABAT活力的影响。结果　在体外 ,终浓度为 10 -9～ 10 -4mol/L的DM或PM对大鼠大脑皮层、海马、纹状体和小脑的GABAT活力无明显影响 ;一次性经口给予 37.5mg/kg的DM组大鼠大脑皮层、海马和小脑的GABAT活力分别为 (2 .96± 0 .4 3)、(2 .13± 0 .4 4 )、(5 .12± 1.36 )nmol·mgpro-1·min-1,低于对照组 (3.4 3± 0 .4 1)、(2 .6 8± 0 .4 7)、(6 .74± 1.6 4 )nmol·mgpro-1·min-1],差异有显著性 (P <0 .0 5 ) ,6 0 0mg/kgPM一次性染毒组大鼠大脑皮层和海马的GABAT活力分别为(4.5 7± 0 .30 )、(4.18± 0 .6 3)nmol·mgpro-1·min-1,高于对照组 ,差异有显著性 (P <0 .0 5 ) ;连续 5d每天 1次经口给予 12 .5mg/kg的DM或 2 0 0mg/kg的PM ,均对大鼠大脑皮层、海马、纹状体和小脑的GABAT活力无明显影响。结论　在体外 ,DM和PM均对大鼠脑组织GABAT活力无明显影响 ;在整体实验中 ,DM和PM对大鼠脑组织GABAT活力的影响可能存在差异。

Effects of pyrethroids on the activity of γ-aminobutyric acid transferase in rat brain

OBJECTIVE: To study the effects of pyrethroids on the activity of gamma-aminobutyric acid transferase (GABAT) in rat brain. METHOD: The coupled enzyme ultraviolet spectrophotography was applied to observe the effects of deltamethrin (DM) and permethrin (PM) on the activities of GABAT in rat cerebral cortex, hippocampus, corpus striatum and cerebellum in vitro and in vivo. RESULTS: In vitro, DM and PM had no significant effects on the activities of GABAT in rat cerebral cortex, hippocampus, corpus striatum and cerebellum at the final concentration of 10(-9) - 10(-4) mol/L. When 37.5 mg/kg DM and 600 mg/kg PM were orally administrated to the rats at one time, the activities of GABAT in rat cerebral cortex, hippocampus and cerebellum in the DM group (2.96 +/- 0.43), (2.13 +/-0.44), (5.12 +/- 1.36) nmol x mg pro(-1) x min(-1), respectively] were lower than those in the control group (3.43 +/- 0.41), (2.68 +/- 0.47), (6.74 +/- 1.64) nmol x mg pro(-1) x min(-1)] (P < 0.05), and the activities of GABAT in rat cerebral cortex and hippocampus in the PM group (4.57 +/- 0.30), (4.18 +/- 0.63) nmol.mg pro(-1) x min(-1), respectively] were higher than those in the control group (P < 0.05). When 12.5 mg/kg DM and 200 mg/kg PM were orally administrated to the rats once a day for consecutive five days, the two pesticides had no significant effects on the activities of GABAT in rat cerebral cortex, hippocampus, corpus striatum and cerebellum (P > 0.05). CONCLUSIONS: In vitro, DM and PM had no significant effects on the activity of GABAT in rat brain; in vivo, DM and PM may have different effects on the activity of GABAT in rat brain, which deserve further study.