二氧化硅提取核酸用于多聚酶链反应

利用硫氰酸胍裂解病毒和二氧化硅颗粒能够特异性吸附核酸的特性，将二氧化硅悬液、裂解液及肝炎病毒感染血清共同保温，病毒裂解释放出的核酸结合在二氧化硅颗粒上，经过双蒸水洗脱，所得核酸直接作ＰＣＲ扩增。

PURIFICATION OF NUCLEIC ACIDS BY USING SILICA FOR POLYMERASE CHAIN REACTION

A rapid and sensitive protocol for purification of nucleic acids(DNA and RNA) from serum is reported.The method is based on the lysing property of guanidinium thiocyanate together with the nucleic acids-binding property of silica.The silica particals were mixed both with splitting buffer and HBV/HCV infected serum in 1.5ml eppendorf reaction tube at room temperature.The released nucleic acids of virions were bound to the silica particals and subsquently eluted in a low salt buffer.The purified nucleic acid was a good template for PCR.Using this procedure,32 sera being positive to HBsAg,HBeAg and anti-HBc were tested with PCR and HBV DNA was detected in 96%;110 sera being positive to anti-HCV were tested with RT-PCR and HCV RNA was picked up in 82.7%.