聚合酶链反应诊断单纯疱疹病毒性脑炎

用聚合酶链反应（ＰＣＲ）扩增患者脑脊液（ＣｈＦ）中病毒特异性ＤＮＡ可早期快速诊断单纯疱疹病毒性脑炎（ＨＳＥ）。１９例临床确诊的病毒性脑炎患者，经ＰＣＲ在ＣＳＦ中检出单纯疱疹病毒（ＨＳＶ）１３例，全部阳性标本均经分子杂交证实为ＨＳＶ－ＤＮＡ，而１４例其他神经疾病（ＯＮＤ）对照组均为阴性，显示了这一方法的特异性。其中７例病毒性脑炎ＣＳＦ标本分别用ＰＣＲ分子杂交和病毒分离等三种方法检测ＨＳＶ；显示ＰＣＲ最为敏感。表明ＰＣＲ技术的广泛应用将提高ＨＳＥ的早期诊断水平，指导临床正确治疗。

Preliminary study on diagnosis of herpes simplex virus eucephalitisby polymerase chain reaction

To develop an early diagnostic tool fore herpes simplex virus encephalitis(HSE), a polymerase chain reaction(PCR) assay was used fore detection of herpes simplex virus (HSV) DNA in cerebrospinal fluid (CSF). Nineteen of CSF specimens from patients with clinically definite viral encephalitis and 14 of CSF specimens from patients with other neurological diseases (OND) were examined by PCR. Thirteen cases (68%) of positive HSV were found in 19 patients with viral encephalitis, indicating HSE is viral encephalitis that was most frequent encountered incliulcal practice. HSV-DNA was demonstrated by dot hybridization in PCR amplication products of the positive CSF specimens,and' negative HSV was shown on PCR in control patients with OND. Therefore, PCR is a good method with highly specificity for dedecting HSV-DNA. Moreover, HSV was simultaneously examined by PCR, dot hybridization and virus isoiation in 7 cases of viral eneephalitis, the HSV was found in 5, 3 and 2 CSF specimens by the methods respectively. The results reveal that PCR is highly sensitive. It is expected that the wide spread use of PCR will greatly improve the early diagnosis of HSE and appropriate therapy.