| 肝纤维化大鼠肝组织基质金属蛋白酶9及其抑制因子1基因表达的动态变化及下瘀血汤对其影响 |
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| 引用本文： | 都广礼,陈德兴,张艳,韩琳.肝纤维化大鼠肝组织基质金属蛋白酶9及其抑制因子1基因表达的动态变化及下瘀血汤对其影响[J].中国中西医结合消化杂志,2010,18(1). |
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| 作者姓名： | 都广礼 陈德兴 张艳 韩琳 |
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| 作者单位： | 上海中医药大学中药学院,方剂教研室,上海,201203 |
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| 基金项目： | 国家自然科学基金资助项目(30400579) |
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| 摘 要： | 目的]研究免疫性肝纤维化大鼠肝组织基质金属蛋白酶9(MMP-9 mRNA)和基质金属蛋白酶组织抑制因子1(TIMP-1 mRNA)基因表达的动态变化及下瘀血汤对其影响,探讨下瘀血汤抗肝纤维化的机制。方法]猪血清腹腔注射复制大鼠肝纤维化模型,造模8周后灌胃给予下瘀血汤流浸膏干预治疗,用药4周后和造模过程中的1、2、4、8周动态处死大鼠,获取标本,检测指标。结果]与正常组相比,模型组1、8、12周MMP-9 mRNA表达明显降低(P0.05,0.01),2周MMP-9 mRNA表达显著升高(P0.01),4周表达无明显变化。与模型组12周相比,下瘀血汤组MMP-9 mRNA表达明显升高(P0.05)。与正常组相比,1、2、4、8和12周模型组TIMP-1 mRNA表达均有显著升高(P0.05,0.01))。与12周模型组相比,下瘀血汤组TIMP-1 mRNA表达显著降低(P0.01)。结论]大鼠猪血清免疫性肝纤维化存在着MMP-9 mRNA和TIMP-1 mRNA的动态变化,下瘀血汤可通过促进MMP-9 mRNA表达和抑制TIMP-1 mRNA表达而发挥抗肝纤维化的作用。
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| 关 键 词： | 肝纤维化 基质金属蛋白酶9 基质金属蛋白酶组织抑制因子1 基因表达 下瘀血汤 |
Dynamic gene expression of matrix metalloproteinases-9 and tissue inhibitor of metalloproteinase-1 in rat fibrotic liver tissue and effect of Xiayuexue Decoction |
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| DU Guang-li,CHEN De-xing,ZHANG Yan,HAN Lin.Dynamic gene expression of matrix metalloproteinases-9 and tissue inhibitor of metalloproteinase-1 in rat fibrotic liver tissue and effect of Xiayuexue Decoction[J].Chinese Journal of Integrated Traditional and Western Medicine on Digestion,2010,18(1). |
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| Authors: | DU Guang-li CHEN De-xing ZHANG Yan HAN Lin |
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| Institution: | DU Guang-li,CHEN De-xing,ZHANG Yan,HAN Lin(School of Pharmacy,Shanghai University of TCM,Shanghai,201203,China) |
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| Abstract: | Objective]To investigate the changes of mRNA expression of matrix metalloproteinases-9(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP-1)in fibrotic liver tissue induced by porcine serum and effect of Xiayuxue Decoction,so as to highlight anti-fibrotic mechanism of Xiayuxue Decotion.Methods]Liver fibrosis in rats was induced by intraperitoneal injection of porcine serum.After 8wk of modeling,rats were given Xiayuxue Decoction for 4 weeks.Rats were sacrificed and sample was obtained for determinatio... |
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| Keywords: | liver fibrosis MMP-9 TIMP-1 gene expression Xiayuxue Decoction |
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