新疆维汉妇女HR-HPV载量与不同宫颈病变的相关性研究

目的:探讨高危人乳头瘤病毒(HR-HPV)在新疆维汉妇女不同宫颈病变的感染现状,分析其载量与不同宫颈病变的相关性。方法:对202例汉族妇女和160例维吾尔族妇女采用第二代杂交捕获(Hybrid capture,HC2)检测HR-HPV含量,标本的相对荧光光度值(RLU)与阳性定标域值(CO)的比值相当于标本中检出的病毒DNA负荷量(pg/ml)。结果:①在202例汉族患病妇女中,HR-HPV阳性者151例,阴性者51例;其中慢性宫颈炎HR-HPV阳性率为52.8%,CIN1、CIN2～3、宫颈鳞癌(SCC)患者HR-HPV阳性率分别为73.3%、91.6%、94.8%;在160例维吾尔族患病妇女中,HR-HPV阳性者90例,阴性者70例;其中慢性宫颈炎HR-HPV阳性率为35.2%,CIN1、CIN2～3、SCC患者HR-HPV阳性率分别为50.0%、74.3%、82.1%;认为HR-HPV感染率在维汉妇女不同级别宫颈病变有统计学差异(P<0.05);②HR-HPV载量在维吾尔族妇女不同宫颈病变组(慢性宫颈炎、CIN1、CIN2～3和SCC)的中位数为0.92、6.98、52.2和89.6;汉族妇女不同宫颈病变组的中位数为1.26、10.69、82.9和112,维汉妇女不同程度的宫颈病变HR-HPV载量之间有差异(P<0.05)。对同等级别宫颈病变中维吾尔族和汉族妇女HR-HPV载量检测,在慢性炎组差异无统计学意义(P>0.05);在CIN和SCC组均有统计学差异(P<0.05);可以认为维吾尔族和汉族妇女在CIN组和SCC组HR-HPV载量差异有统计学意义,且汉族妇女病毒载量高于维吾尔族妇女。③随着病毒载量的增加,维汉族妇女发生宫颈各级病变的风险性增加,两者之间存在明显的剂量反应关系。结论:HR-HPV感染是导致宫颈病变的主要因素。维汉妇女HPV病毒载量与宫颈病变之间存在一定的剂量反应关系。

The correlation of HR-HPV load capacity and different cervical lesions of Uigur and Han women in Xinjiang

Objective:To discusses the infection present of high-risk human papibna virus(HR-HPV) in different cervical lesions of Uigur and Han women in Xinjiang,and to analyze the correlation of HR-HPV load capacity and different cervical lesions.Methods:The HR-HPV contents of 202 Han women and 160 Uigur women were detected by hybrid-capture 2(HC2).Results:①In 202 HAn women,HR-HPV was positive for 151 cases and was negative for 51 cases;the positive rate of HR-HPV was 52.8%,73.3%,91.6%,94.8% for chronic cervicitis,CINⅠ,CINⅡ-Ⅲ,SCC respectively.In 160 Uigur women,HR-HPV was positive for 90 cases and was negative for 70 cases;the positive rate of HR-HPV was 35.2%,50.0%,74.3%,82.1% for chronic cervicitis,CINⅠ,CINⅡ-Ⅲ,SCC respectively.The positive rates of HR-HPV between Uigur and Han women had statistics difference at different cervical lesions(P<0.05),②The median of HR-HPV load capacity in different cervical lesions group(chronic cervicitis,CINⅠ,CINⅡ-Ⅲ and SCC) was 0.92,6.98,52.2 and 89.6 for Uigur women respectively and was 1.26,10.69,82.9 and 112 for Han women respectively,the differences were significant(P<0.05).In same level cervical lesions,the HR-HPV load capacities between Uigur women and Han women there were no significance differences for the chronic cervicitis group(P>0.05),but there were significance differences for CIN group and SCC group(P<0.05),the HR-HPV load capacities of Han women were higher than Uigur women.③Along with the increasing of HR-HPV load capacities,the possibility of cervical lesions increased for Uigur women and Han women.Conclusion:The HR-HPV infection is a mine factor of cervical lesions There is the dose-response relationship between HR-HPV load capacity and cervical lesions.