VEGFR-3、podoplanin和CD34标记阳性脉管在宫颈癌中的表达及意义

目的:研究VEGFR-3、podoplanin和CD34标记的阳性脉管在宫颈癌组织中表达的特点,分析其与肿瘤临床病理特点之间的关系。方法:采用双标及单标免疫组化方法检测58例宫颈鳞癌组织VEGFR-3、podoplanin和CD34标记阳性脉管的密度。结果:癌灶边缘组织VEGFR-3和podoplanin阳性脉管密度显著高于癌组织(P<0.05),也显著高于对照组宫颈组织(P<0.05);癌灶边缘组织和癌组织CD34阳性脉管密度差异无统计学意义(P>0.05),均高于对照组宫颈组织(P<0.05)。Ⅱa期组癌灶边缘组织VEGFR-3、podoplanin和CD34阳性脉管密度分别为17.46±2.65、14.36±1.21、17.98±4.13显著高于Ⅰb期组的13.52±1.23、10.58±3.25、13.75±3.38(P<0.05);淋巴结转移组癌灶边缘组织VEGFR-3和podoplanin阳性脉管密度18.68±0.89和14.67±3.72显著高于无淋巴结转移组11.45±3.56和10.77±2.65(P<0.05),CD34阳性脉管密度在两组中表达差异无统计学意义(P>0.05)。结论:宫颈癌VEGFR-3、podoplanin和CD34表达与肿瘤临床分期有关,癌灶边缘组织VEGFR-3和podoplanin高表达与淋巴结转移亦密切相关,podoplanin有望成为宫颈癌淋巴结转移预后的一种可靠标志物。

Expressions and significances of VEGFR-3,podoplanin,and CD34 labeled positive vessels in cervical cancer

Objective:To research the characteristics of expressions of endothelial growth factor receptor-3(VEGFR-3),podoplanin,and CD34 labeled positive vessels in cervical cancer tissue,analyze the relationship between their expressions and clinicopathological characteristics of cervical cancer.Methods:Double-labeling and single-labeling immunohistochemistry were used to detect the densities of VEGFR-3,podoplanin,and CD34 labeled positive vessels in 58 cases with cervical cancer.Results:The densities of VEGFR-3 and podoplanin labeled positive vessels in adjacent tissue of cervical cancer were significantly higher than those in cervical cancer tissue(P<0.05) and cervical tissue in control group(P<0.05);there was no statistically significant difference in the density of CD34 labeled positive vessels between adjacent tissue of cervical cancer and cervical cancer tissue(P>0.05);the densities of CD34 labeled positive vessels in adjacent tissue of cervical cancer and cervical cancer tissue were significantly higher than that in cervical tissue in control group(P<0.05).The densities of VEGFR-3,podoplanin,and CD34 labeled positive vessels in adjacent tissue of cervical cancer of stage Ⅱa were(17.46±2.65),(14.36±1.21),and(17.98±4.13),respectively,which were significantly higher than those in adjacent tissue of cervical cancer of stage Ⅰb((13.52±1.23),(10.58±3.25),and(13.75±3.38),respectively)(P<0.05).The densities of VEGFR-3 and podoplanin in adjacent tissue of cervical cancer in lymph node metastasis group were(18.68±0.89) and(14.67±3.72),respectively,which were significantly higher than those in adjacent tissue of cervical cancer in non-lymph node metastasis group((11.45±3.56) and(10.77±2.65),respectively)(P<0.05),there was no statistically significant difference in the density of CD34 labeled positive vessels between the two groups(P>0.05).Conclusion:The expressions of VEGFR-3,podoplanin,and CD34 in cervical cancer are correlated with clinical stage of cervical cancer,the high expressions of VEGFR-3 and podoplanin in adjacent tissue of cervical cancer are related to lymph node metastasis closely,podoplanin could become to be a reliable marker to predict lymph node metastasis and prognosis of cervical cancer.