山茶籽联合雌二醇对去卵巢大鼠骨重建和骨代谢酶的影响

目的:了解山茶籽联合雌二醇对去卵巢大鼠骨重建和骨代谢酶的影响,为山茶籽联合雌二醇治疗Ⅰ型骨质疏松症提供实验依据。方法:将90只5月龄健康雌性大白鼠分成假手术组(sham)、去卵巢模型组(OVX)、山茶籽组、雌二醇组(E2)、小剂量山茶籽+雌二醇组(Ts+E2),每组各18只。各实验组在第8、12、16周,随机处死6只大鼠,取左股骨切片观察骨组织,取右股骨测量骨密度,取左心血测量血清雌二醇、碱性磷酸酶。数据进行统计学分析。结果:OVX组的血清雌二醇和骨密度明显低于sham组(P<0.01),而血清碱性磷酸酶明显高于sham组(P<0.01);3个治疗组与sham组相比,各时间的血清雌二醇、碱性磷酸酶、骨密度均无显著性差异(P>0.05)。结论:小剂量的雌二醇联合山茶籽对去卵巢大鼠的骨质疏松症的治疗效果与单独使用较大剂量的山茶籽或较大剂量的雌二醇的治疗效果相近。

Affect of Mountain tea seed combined Estradiol on bone remodeling in ovariectomized rats and bone metabolic enzymes

Objective:To understand the affect of Mountain tea seed combined Estradiol on bone remodeling in ovariectomized rats and bone metabolic enzymes,and to provide experimental basis for the treatment of type Ⅰosteoporosis by Mountain tea seed combined Estradiol.Methods:5-month-old healthy female rats 90 were divided into five experimental groups(n=18):① sham operation group(sham);② model group,ovariectomized(OVX);③ Mountain tea seed group(Ts),mountain water-soluble alcohol extract tea seed fed,10 ml/kg.d(quite crude drug 5 g/g);④ Estradiol(E2),subcutaneous injection of Estradiol 200μg/kg,2 times / week;⑤ Mountain tea seed + low-dose Estradiol group(Ts + E2),subcutaneous injection of Estradiol 100 μg/kg,2 times / week and Mountain tea seed extract water-soluble alcohol fed,10 ml/kg.d(quite crude drug 2.5 g/g).At 8,12 and 16 weeks,the experimental groups were randomly killed six rats,the left femur bone tissue slices were observed,the density measurements of right femur bone were tested,serum Estradiol and alkaline phosphatase to take the left were tested.Data were statistically analyzed.Results:In OVX group,serum Estradiol and bone mineral density was significantly lower than the sham group(P<0.01),while serum alkaline phosphatase was significantly higher than the sham group(P<0.01);comparing with the sham group,Estradiol,alkaline phosphatase,bone density were no significant difference in the three treatment groups(P>0.05).Conclusion:The clinical effects of small doses of Estradiol and Mountain tea seed on the osteoporosis of ovariectomized rats is similar with large doses of Mountain tea seed or Estradiol.