基于网络药理学的“苍术-玄参”药对抗2型糖尿病作用机制研究

目的采用网络药理学方法研究苍术-玄参药对抗2型糖尿病的作用机制。方法通过检索中药系统药理数据分析平台(TCMSP),获得苍术、玄参主要活性成分和相关作用靶点,并借助Cytoscape 3.6.1软件构建苍术-玄参药对活性成分-靶点网络图。通过TTD数据库中检索抗2型糖尿病靶点,在STRING数据库进行蛋白质间相互作用分析,构建抗2型糖尿病靶点PPI网络图,利用Cytoscape 3.6.1软件中的Merge功能与活性成分-靶点融合,获得苍术-玄参药对抗2型糖尿病作用靶点。运用DAVID数据库对苍术-玄参药对抗2型糖尿病作用靶点进行KEGG通路富集,进一步研究苍术-玄参药对抗2型糖尿病作用机制。结果获得具有类药性、口服吸收良好的成分27个,苍术-玄参药对抗2型糖尿病作用靶点10个,包括PTGS2、DPP4、PTGS1、NR3C2等。KEGG分析得到与苍术-玄参药对抗2型糖尿病作用有关的通路6条,关键通路包括脂肪细胞脂解调控通路、PPAR信号通路、胰岛素抵抗、AMPK信号通路等。结论苍术-玄参中的活性物质主要通过作用于PTGS2、DPP4、PTGS1、NR3C2等靶点协同治疗2型糖尿病,为深入剖析苍术-玄参药对复杂体系,阐释其治疗2型糖尿病的潜在复杂作用机制提供了新方向。

Study on Mechanism of Atractylodis Rhizoma-Scrophulariae Radix Drug Pair Against Type 2 Diabetes Mellitus Based on Network Pharmacology

OBJECTIVE To explore the mechanism of Atractylodis Rhizoma-Scrophulariae Radix drug pair against type 2 diabetes mellitus using network pharmacology. METHODS The chemical components and targets of Atractylodis Rhizoma-Scrophulariae Radix were searched from TCMSP database and Cytoscape 3.6.1 was used to build a network between components and targets. Secondly, "type 2 diabetes mellitus" targets were searched form TTD, and targets retrieved were used to build a PPI network based on the analysis by STRING database. To obtain type 2 diabetes mellitus targets of the active components, the PPI network was fused with the component-target network. Finally, the DAVID database was used to perform KEGG pathway enrichment analysis in order to explore the mechanism of Atractylodis Rhizoma-Scrophulariae Radix drug pair against type 2 diabetes mellitus. RESULTS The 27 kinds of effective compounds were obtained, 10 target proteins and 6 enrichment pathways were selected. Key targets included PTGS2, DPP4, PTGS1, NR3C2, etc. Regulation included regulation of lipolysis in adipocytes, PPAR signaling pathway, insulin resistance, AMPK signaling pathway, etc. CONCLUSION The active components of Atractylodis Rhizoma-Scrophulariae Radix act on insulin receptor in the treatment of type 2 diabetes mainly through PTGS2, DPP4, PTGS1, NR3C2, etc. This study provide a new foundation for further study on the mechanism of Atractylodis Rhizoma-Scrophulariae Radix composition.