Myelin phagocytosis in Wallerian degeneration |
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Authors: | P Scheidt R L Friede |
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Institution: | (1) Abteilungen für Neurologie, Universtität Göttingen, Robert-Koch-Strasse 40, D-3400 Göttingen, Federal Republic of Germany;(2) Abteilungen für Neuropathologie, Universtität Göttingen, Robert-Koch-Strasse 40, D-3400 Göttingen, Federal Republic of Germany |
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Abstract: | Summary Myelin phagocytosis in Wallerian degeneration was studied using a model of murine sciatic nerve degeneration in millipore diffusion chambers in the peritoneal cavity of host mice. Immunocytological investigations showed the dependence of myelin digestion on the invasion of Fc-positive, Mac-1-positive and partly Ia-positive monocytes. Lymphocytes did not play a prominent role. Compared to Wallerian degeneration in situ, phagocytosis was decreased in nerves enclosed by millipore membranes on both sides of the chamber. The membrane acted as a trap for invading monocytes/macrophages. Neither tissue integrity nor genetic strain influenced the degree of phagocytosis. A modification of the experimental technique is introduced which permits myelin phagocytosis in the peritoneal cavity in a degree comparable to that in Wallerian degeneration in situ.Supported by a grant from the Deutsche Forschungsgemeinschaft (609) |
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Keywords: | Wallerian degeneration Myelin phagocytosis Macrophages Millipore diffusion chambers Immunocytochemistry |
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