A method for coupling cytomegalovirus antigens to aldehyde-fixed erythrocytes for use in passive hemagglutination |
| |
| Authors: | H Ikram A M Prince |
| |
| Affiliation: | The Lindsley F. Kimball Research Institute of The New York Blood Center, 310 East 67th Street, New York, NY 10021, U.S.A. |
| |
| Abstract: | A method for coupling cytomegalovirus (CMV) antigens to aldehyde-fixed erythrocytes for use in a passive hemagglutination assay for antibody to CMV is described. This method uses acid buffer instead of tannic acid for coupling CMV-complement fixation (CF) antigen to the stabilized cells. The coated cells are stable for at least 6 mth at -70 degrees C and for 5 days at 4 degrees C. This provides an inexpensive reagent having a sensitivity for anti-CMV detection which compares favorably with that of the CF test and enzyme immunoassay. |
| |
| Keywords: | cytomegalovirus antigens aldehyde-fixed erythrocytes passive hemagglutination |
| 本文献已被 ScienceDirect 等数据库收录！ |
